Tissue Factor Pathway Inhibitor-2 Silencing Promotes Hepatocellular Carcinoma Cell Invasion In Vitro
Article first published online: 24 OCT 2013
Copyright © 2013 Wiley Periodicals, Inc.
The Anatomical Record
Volume 296, Issue 11, pages 1708–1716, November 2013
How to Cite
Zhu, B., Zhang, P., Zeng, P., Huang, Z., Dong, T.-F., Gui, Y.-K. and Zhang, G.-W. (2013), Tissue Factor Pathway Inhibitor-2 Silencing Promotes Hepatocellular Carcinoma Cell Invasion In Vitro. Anat Rec, 296: 1708–1716. doi: 10.1002/ar.22789
- Issue published online: 24 OCT 2013
- Article first published online: 24 OCT 2013
- Manuscript Accepted: 30 JUN 2013
- Manuscript Received: 10 FEB 2013
- tissue factor pathway inhibitor-2;
- hepatocellular carcinoma;
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death in the world and metastasis is an essential aspect of HCC progression. Tissue factor pathway inhibitor-2 (TFPI-2) has been implicated as a potential suppressor gene to regulate tumor invasion and metastasis. In this study, we silenced TFPI-2 in the HCC cell line MHCC97-L and evaluated the role of TFPI-2 in cell invasion and its impact on gene expression. We showed in this study that stable TFPI-2 downregulation in MHCC97-L cells resulted in increased cell adhesion and invasion. We also showed that mRNA and protein expression levels of MMP-1/3, CD44, and ICAM-1 were increased, while those of MMP-2/9 were not changed by TFPI-2 silencing. Furthermore, silencing of TFPI-2 caused increased Akt phosphorylation level and NF-κB transcription in MHCC97-L cells. In conclusion, this study confirms that TFPI-2 downregulation can contribute to tumor invasion of HCC cells through alteration in the expression of metastasis-related genes. Anat Rec, 296:1708–1716, 2013. © 2013 Wiley Periodicals, Inc.