Paneth Cell Identification in the Small Intestine of Guinea Pig Offsprings (Cavia porcellus)

Authors

  • María Elith Vásquez Cachay,

    Corresponding author
    1. Animal Physiology Laboratory, Veterinary Medicine School, National University of San Marcos, San Borja, Lima, Perú
    • Correspondence to: María Vásquez Cachay; Veterinary Medicine School, National University of San Marcos, Av. Circunvalación 2800, San Borja, Lima, Perú. E-mail: evasquezc@gmail.com

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  • Erika Pebe Gomez,

    1. Animal Physiology Laboratory, Veterinary Medicine School, National University of San Marcos, San Borja, Lima, Perú
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  • José Luis Rodríguez Gutiérrez,

    1. Experimental Centre IVITA-El Mantaro, Veterinary Medicine School, National University of San Marcos, San Borja, Lima, Perú
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  • Boris Lira Mejía,

    1. Animal Physiology Laboratory, Veterinary Medicine School, National University of San Marcos, San Borja, Lima, Perú
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  • Néstor Falcón Pérez,

    1. Veterinary and Zootecnic Medicine School, Cayetano Heredia University (UPCH), San Martin de Porres, Lima, Perú
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  • Carolina Natalia Zanuzzi,

    1. Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina
    2. National Scientific and Technical Research Council (CONICET-CCT La Plata), Buenos Aires, Argentina
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  • Claudio Barbeito

    1. Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina
    2. National Scientific and Technical Research Council (CONICET-CCT La Plata), Buenos Aires, Argentina
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ABSTRACT

The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth. Anat Rec, 297:856–863, 2014. © 2014 Wiley Periodicals, Inc.

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