Paneth Cell Identification in the Small Intestine of Guinea Pig Offsprings (Cavia porcellus)
Article first published online: 12 FEB 2014
Copyright © 2014 Wiley Periodicals, Inc.
The Anatomical Record
Volume 297, Issue 5, pages 856–863, May 2014
How to Cite
Vásquez Cachay, M. E., Gomez, E. P., Rodríguez Gutiérrez, J. L., Lira Mejía, B., Pérez, N. F., Zanuzzi, C. N. and Barbeito, C. (2014), Paneth Cell Identification in the Small Intestine of Guinea Pig Offsprings (Cavia porcellus). Anat Rec, 297: 856–863. doi: 10.1002/ar.22890
- Issue published online: 22 APR 2014
- Article first published online: 12 FEB 2014
- Manuscript Accepted: 8 JAN 2014
- Manuscript Received: 15 APR 2013
- National University of San Marcos
- Paneth cells;
- guinea pig;
The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth. Anat Rec, 297:856–863, 2014. © 2014 Wiley Periodicals, Inc.