Wenjun Liao and Li Sun contributed equally to this work.
LC3A-Positive “Stone-Like” Structures Predict an Adverse Prognosis of Gastric Cancer
Article first published online: 14 FEB 2014
Copyright © 2014 The Authors The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology Published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
The Anatomical Record
Volume 297, Issue 4, pages 653–662, April 2014
How to Cite
Liao, W., Sun, L., Wang, C., Huang, H., Liu, J., Liao, W. and Shi, M. (2014), LC3A-Positive “Stone-Like” Structures Predict an Adverse Prognosis of Gastric Cancer. Anat Rec, 297: 653–662. doi: 10.1002/ar.22895
- Issue published online: 18 MAR 2014
- Article first published online: 14 FEB 2014
- Manuscript Accepted: 14 JAN 2014
- Manuscript Revised: 8 JAN 2014
- Manuscript Received: 28 MAY 2013
- Science and Technology Program Planning Project of Guangdong Province . Grant Number: 2011B031800281
- Wu Jieping Medical Foundation . Grant Number: 3206740.10017
- gastric cancer;
- stone-like structure;
Microtubule-associated protein light chain 3 (LC3A) is a reliable marker of autophagy that displays three distinct patterns of immunohistochemical staining in solid tumors: diffuse cytoplasmic staining, juxtanuclear staining, and staining of “stone-like” structures. These three patterns have a different prognostic significance in many solid tumors, but little is known about their influence in gastric cancer (GC). This study was a retrospective analysis of 188 GC patients from stages I to IV. The pattern of LC3A expression was examined in tumor and nontumor tissues by immunohistochemistry. Then, the association between the pattern of LC3A expression in GC and the prognosis was investigated by Kaplan-Meier analysis and the Cox proportional hazards model. Two distinct patterns of LC3A immunostaining (diffuse cytoplasmic expression and “stone-like” structures) were observed in GC tissues. LC3A-positive “stone-like” structures were found only in the tumors, and the number of such structures was correlated with both the tumor type and tumor stage. In addition, a high number of LC3A-positive “stone-like” structures was closely associated with an increased risk of recurrence after radical resection of stages I–III cancer (P < 0.001; HR = 0.205) and was associated with a lower overall survival rate for stage IV cancer (P < 0.001; HR = 0.364). Taken together, our data demonstrate that LC3A-positive “stone-like” structures can be used as an independent biomarker for an adverse prognosis of GC, suggesting that “stone-like” structures are correlated with the malignancy of this disease. Anat Rec, 297:653–662, 2014. © 2014 The Authors The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology Published by Wiley Periodicals, Inc.