Tissue alterations in urethral and vaginal walls related to multiparity in rabbits

Authors

  • Nicte Xelhuantzi,

    1. Doctorado en Ciencias Naturales, Universidad Autónoma de Tlaxcala, Tlaxcala, México
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  • Jorge Rodríguez-Antolín,

    1. Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
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  • Leticia Nicolás,

    1. Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
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  • Francisco Castelán,

    1. Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
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  • Estela Cuevas,

    1. Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
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  • Margarita Martínez-Gómez

    Corresponding author
    1. Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
    2. Depto. de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), México DF, México
    • Correspondence to: M. Martínez-Gómez, Departamento de Biología Celular y Fisiología. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México. Unidad Periférica Tlaxcala. Carretera Tlaxcala-Puebla Km. 1.5, C.P. 90070, Tlaxcala, México. Fax +52–246-46-215-57. E-mail: marmag@biomedicas.unam.mx

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ABSTRACT

In rodents, vaginal distention after delivery or experimental manipulation affects innervations as well as the amount of striated/smooth musculature or collagen in both the urethra and vagina. These changes are associated with modifications in excretory and reproductive processes. Although successive and consecutive vaginal deliveries (multiparity) affect the contractile and functional properties of the female lower urogenital tract (LUT), its impact on LUT morphometry, including persistency, has been barely studied. The caudal urethra (CU) and cranial (V1) and caudal (V2) pelvic vaginal regions were excised from young and adult nulliparous (YN and AN, respectively) and multiparous (YM and AM, respectively) rabbits. Tissues were histologically processed and stained with Masson's trichrome. The thickness of the tissue layers and areas covered by tissue components were measured and compared using two-way ANOVA followed by Student-Newmann-Keuls tests to determine statistical differences (P ≤ 0.05). Compared to YN, YM, and AN tissues showed a reduction in the thickness of the epithelium, as well as in areas covered by striated musculature, collagen, and blood vessels of the LUT. In comparison with YM, only some morphometric changes were recovered in the AM group. Our study shows that multiparity and age can be associated with epithelial and muscular atrophy of urethral and vaginal walls. The morphometry of the LUT between young and adult female rabbits varies with multiparity. These findings may help to better understand the effects of multiparity on young and adult females and its correlation with the development of pelvic dysfunctions. Anat Rec, 297:1963–1970, 2014. © 2014 Wiley Periodicals, Inc.

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