Bovine circumvallate taste buds: Taste cell structure and immunoreactivity to α-gustducin
Article first published online: 27 JAN 2003
Copyright © 2003 Wiley-Liss, Inc.
The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume 271A, Issue 1, pages 217–224, March 2003
How to Cite
Tabata, S., Wada, A., Kobayashi, T., Nishimura, S., Muguruma, M. and Iwamoto, H. (2003), Bovine circumvallate taste buds: Taste cell structure and immunoreactivity to α-gustducin. Anat. Rec., 271A: 217–224. doi: 10.1002/ar.a.10028
- Issue published online: 27 JAN 2003
- Article first published online: 27 JAN 2003
- Manuscript Accepted: 21 OCT 2002
- Manuscript Received: 3 JUN 2002
- circumvallate papilla;
- taste bud cell;
- transmission electron microscopy;
The taste buds of bovine circumvallate papillae were investigated under light and electron microscopy both by histological and immunohistochemical methods. Taste buds existed in the inner epithelium of the trench of the papillae. Under electron microscopy, two types of taste cells, type I and type II, could be classified according to the existence of dense-cored vesicles and cytoplasmic density. Type I had electron-lucent cytoplasm and possessed many electron-dense cored vesicles in the apical cytoplasm. It was considered that the electron-dense materials of the vesicles were released and constituted the pore substance. This type of cell possessed long and thick apical processes in the taste pore. Type II had denser electron cytoplasm compared with that of type I and possessed many electron-lucent vesicles in the apical cytoplasm. This type of cell possessed microvilli in the taste pore. To know the immunoreactivity to α-gustducin in bovine circumvallate taste buds, we used the immunoblotting method and the immunohistochemical method. The α-gustducin reaction band at 40 kDa was displayed in the specimen of Western blots. The immunohistochemical property of the antiserum to α-gustducin was investigated by using the avidin-biotin complex (ABC) method and the 1.4-nm gold and silver enhancement methods. A subset of taste cells showed the immunoreactivity under light microscopy. The electron microscopic specimens with the 1.4-nm gold and silver enhancement method revealed that only type II cells exhibited the α-gustducin immunoreactivity. Anat Rec Part A 271A:217–224, 2003. © 2003 Wiley-Liss, Inc.