Disruption of actin cytoskeleton and anchorage-dependent cell spreading induces apoptotic death of mouse neural crest cells cultured in vitro

Authors

  • Atsushi Hinoue,

    1. Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    2. Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan
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  • Toshiya Takigawa,

    1. Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto, Japan
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  • Takashi Miura,

    1. Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto, Japan
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  • Yoshihiko Nishimura,

    1. Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan
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  • Shigehiko Suzuki,

    1. Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan
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  • Kohei Shiota

    Corresponding author
    1. Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    2. Congenital Anomaly Research Center, Kyoto University Graduate School of Medicine, Kyoto, Japan
    • Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan
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    • Fax: 81-75-751-7529


Abstract

In vertebrate embryos, neural crest cells emigrate out of the neural tube and contribute to the formation of a variety of neural and nonneural tissues. Some neural crest cells undergo apoptotic death during migration, but its biological significance and the underlying mechanism are not well understood. We carried out an in vitro study to examine how the morphology and survival of cranial neural crest (CNC) cells of the mouse embryo are affected when their actin cytoskeleton or anchorage-dependent cell spreading is perturbed. Disruption of actin fiber organization by cytochalasin D (1 μg/ml) and inhibition of cell attachment by matrix metalloproteinase-2 (MMP-2; 2.0 units/ml) were followed by morphologic changes and apoptotic death of cultured CNC cells. When the actin cytoskeleton was disrupted by cytochalasin D, the morphologic changes of cultured CNC cells preceded DNA fragmentation. These results indicate that the maintenance of cytoskeleton and anchorage-dependent cell spreading are required for survival of CNC cells. The spatially and temporally regulated expression of proteinases may be essential for the differentiation and migration of neural crest cells. © 2005 Wiley-Liss, Inc.

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