Myocardial heterogeneity in permissiveness for epicardium-derived cells and endothelial precursor cells along the developing heart tube at the onset of coronary vascularization
Version of Record online: 3 JAN 2005
Copyright © 2005 Wiley-Liss, Inc.
The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume 282A, Issue 2, pages 120–129, February 2005
How to Cite
Lie-Venema, H., Eralp, I., Maas, S., Gittenberger-De Groot, A. C., Poelmann, R. E. and Deruiter, M. C. (2005), Myocardial heterogeneity in permissiveness for epicardium-derived cells and endothelial precursor cells along the developing heart tube at the onset of coronary vascularization. Anat. Rec., 282A: 120–129. doi: 10.1002/ar.a.20154
- Issue online: 24 JAN 2005
- Version of Record online: 3 JAN 2005
- Manuscript Accepted: 3 SEP 2004
- Manuscript Received: 22 JUN 2004
- Netherlands Heart Foundation. Grant Number: 2001B015
- coronary development;
- endothelial (precursor) cells;
The coronary vasculature develops from mesothelial and endothelial precursor cells (EPCs) derived from the proepicardial organ (PEO), which migrate over the heart to form the epicardium. By epithelial-mesenchymal transition (EMT), the subepicardium and epicardium-derived cells (EPDCs) are formed. EPDCs migrate into the myocardium, where they differentiate into smooth muscle cells and fibroblasts that stabilize the developing coronary vasculature and contribute to myocardial architecture. Complete PEO ablation results in embryonic lethality due to cardiac defects, including a looping disorder with a too wide inner curvature. To investigate the behavior of early coronary contributors, we analyzed normal quail embryos and found lumenized endothelial vessels in the subepicardium already at stage HH19. Furthermore, EPCs had penetrated into the myocardium of the inner curvature. To confirm that the myocardium of the inner curvature is specifically permissive for EPCs and to study early EPDC migration in more detail, chimeric chicken embryos harboring a quail PEO were analyzed. Lateral epicardial outgrowth and EMT were observed throughout, but migration into the myocardium was restricted to the inner curvature between HH19 and 22. The permissive myocardial area expanded to the atrium, atrioventricular canal, and trabeculated ventricle at stage HH23–24. In contrast, outflow tract myocardium was never found to be permissive for EPDCs and EPCs until HH30, not even when the quail PEO was attached directly onto it. We conclude that early coronary formation starts in the inner curvature and hypothesize that the presence of PEO-derived cells is essential for the maturation of the inner curvature and subsequent looping of the heart tube. © 2005 Wiley-Liss, Inc.
Heart development starts with the formation of a simple tube consisting of an inner endocardial layer and an outer myocardial layer, with cardiac jelly in between. With increase in size and functioning of the heart, the development of a coronary circulation becomes necessary. Coronary formation is classically divided in primary formation of the endothelial network, during the process of angiogenesis, and in a secondary stabilization of the vessel wall by smooth muscle cells (SMCs) and pericytes during the process of arteriogenesis. Both processes are initiated by the development of the proepicardial organ (PEO) and the epicardium during the late looping phase of the tubular heart. The PEO can be seen as villous protrusions from the ventral wall of the sinus venosus and consists of mesothelial, mesenchymal, and endothelial (precursor) cells (Poelmann et al., 1993). From the proepicardial villi, cells transverse the pericardial cavity along extracellular matrix bridges (Nahirney et al., 2003) and attach to the dorsal side of the developing ventricle (Männer, 1992), where they start spreading over the myocardial surface of the heart tube to form the epicardium (Vrancken Peeters et al., 1995). By epithelial-mesenchymal transition (EMT) of epicardial cells, the mesenchymal subepicardium is formed. Cells from this layer, the so-called epicardium-derived cells (EPDCs), migrate and differentiate into coronary smooth muscle cells and adventitial fibroblasts to stabilize the developing coronary vessels (Vrancken Peeters et al., 1999) and into interstitial fibroblasts to give rigidity to the myocardium (Gittenberger-de Groot et al., 1998). Furthermore, they migrate to the endocardial cushions of the atrioventricular canal (Gittenberger-de Groot et al., 1998). The importance of proper development of the (sub)epicardium and hence of correct EPDC deposition and differentiation is underlined by an increasing number of studies in which epicardial development was manipulated, either mechanically (Gittenberger-de Groot et al., 2000) or genetically (Kwee et al., 1995; Yang et al., 1995; Moore et al., 1999; Tevosian et al., 2000; Lie-Venema et al., 2003). The spectrum of cardiac defects ranges from severe abnormalities resulting in embryonic lethality, e.g., with very thin myocardium, absence of coronary development, and abberant cardiac looping in quail embryos with a completely ablated PEO (Gittenberger-de Groot et al., 2000), to milder disturbances as diminished coronary SMC deposition and abnormal patterning of coronary arteries in antisense Ets-treated chicken embryos (Lie-Venema et al., 2003).
Whereas the migration patterns of the proepicardium and their derivative mesenchymal EPDCs have been studied quite extensively (Wessels and Perez-Pomares, 2004), detailed studies on the developmental behavior of the proepicardial endothelial (precursor) population during early epicardial and coronary development have begun to emerge only recently (Kattan et al., 2004). We therefore analyzed the initial events of coronary endothelial (precursor) cell migration at the onset of coronary development.
MATERIALS AND METHODS
Normal and Chimeric Embryos
Normal endothelial (precursor) cell behavior was studied in Japanese quail (Coturnix coturnix japonica) embryos. To study EPDC and endothelial (precursor) cell migration in more detail, chimeras were generated using White Leghorn chicken (Gallus domesticus) embryos as hosts and quail embryos as donors. Embryos were staged according to the criteria of Hamburger and Hamilton (1951).
Quail-chicken chimeras were made basically according to the chimerization technique as described by Poelmann et al. (1993). In brief, the proepicardium of an Hamburger and Hamilton stage 15–18 (HH15–18) quail embryo was isolated together with a tiny piece of liver tissue to provide endothelial precursor cells to the proepicardial transplant. The isolated proepicardium and adjacent liver tissue were transplanted into the pericardial cavity of a HH15–18 chicken host embryo through the naturally occurring hiatus in the body wall that exists until HH18. The transplanted tissue was positioned at different sites along the developing heart tube: adjacent to the sinus venosus and atrial region, in the inner curvature or dorsally of the ventricle (Fig. 1). Chimeras were harvested between stage HH19 and HH30. The number and characteristics of successful chimerizations, verified by a specific quail nuclear immunohistochemical staining with QCPN, are listed in Table 1.
|Harvesting stage (HH)||Stage at time of transplantation (HH)||Transplant site||Attachment sitea|
|Quail PEO||Chicken host|
|19||16||15||IC||A, AVC, OT|
|20||17||15||IC||A, AVC, OT|
|20||16||16||IC||AVC, IC, OT|
|21||15||15||IC||AVC, IC, OT|
|25||17||17||SV||AVC, IC, OT, Li,|
Unless indicated otherwise, tissue processing and immunohistochemistry were as described earlier (Vrancken Peeters et al., 1999). Normal and chimeric embryos were fixed by overnight immersion in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (pH 7.4) at 4°C. Serial sections were immunostained with the monoclonal antibodies QCPN (Hybridoma Bank, Baltimore, MD; diluted 1:4), QH1 (Hybridoma Bank; diluted 1:500), and HHF35 (Dakopatts M635, Glostrup, Denmark; diluted 1:500) that recognize specifically quail cell nuclear antigen, quail endothelial (precursor) cells, and muscle actins, respectively. For the QCPN staining, antigen unmasking was applied by incubating the sections for 3 × 4 min in 0.01 M citrate buffer (pH 6.0) at 98°C in a microwave. Sections were examined by light microscopy. An Olympus AX-70 light microscope in combination with an Olympus DP-12 digital camera was used for microphotography.
The QH1 antibody recognizes specifically quail endothelial cells and their precursors. As was defined earlier (Poelmann et al., 1993), only QH1-positive cells that line a lumen are considered to be true endothelial cells. Dispersed single or clustered cells located in either mesenchymal or mesothelial regions of the (sub)epicardium or the myocardium are considered as endothelial precursor cells when they do not line a lumen, but have already a relatively flat endothelial cell-like appearance. Isolated round and relatively large QH1-positive cells are considered to be cells of the hematopoietic lineage (hemangioblasts) that can give rise to several blood cell types and to endothelial precursor cells (Choi, 2002). QH1 also recognizes endocardial cells. Thus, it is impossible to discern PEO-derived endothelial (precursor) cells from endocardial cells originating from the primary heart field by staining quail embryonic sections with QH1.
PEO, epicardium, subepicardium, and EPDCs.
The PEO is defined as the cauliflower-like protrusion that sprouts from the sinus venosus, traverses the pericardial cavity, and attaches to the myocardium of the ventricle. The PEO contains a variety of cell and tissue types, namely, endothelial (precursor) cells, capillaries, epithelium, and mesenchyme. Once the PEO has attached to the myocardial surface, the outer mesothelial layer is defined as the epicardium. The subepicardial mesenchymal cells, originating from the epicardium by EMT, form the subepicardium. EPDCs encompass all cells derived from the epicardium, that is, the subepicardial mesenchymal cells and their derivatives (smooth muscle cells and interstitial fibroblasts).
Early Endothelial (Precursor) Cell Migration in Normal Quail Embryos
To study the earliest events in coronary formation, we analyzed PEO-associated endothelial cell migration in normal quail embryos between HH18 and HH27. As can be seen in Figure 2A and B, endothelial precursor cells were present in the mesothelial epicardium and in the mesenchymal subepicardium already at stage HH17+. The close juxtaposition of the liver primordium and the proepicardium, clearly visible in transverse sections of HH17 and HH21 embryos (Fig. 2A and D), suggests that the liver, which is a hemangioblastic organ at this age (Wong and Cavey, 1993), is the primary source of these proepicardial endothelial precursor cells. Migration of the EPCs through the proepicardial organ results in the formation of lumenized endothelial capillaries already at stage HH19 (Fig. 2C and D). These early capillaries form in the subepicardium that is located dorsally of the primitive atrioventricular canal and ventricle, at the initial attachment site of the PEO. In this lumenized vessel, network erythrocytes were found only sporadically in six quail embryos (HH20–22; Fig. 2D). Blood vessels containing notable amounts of erythrocytes were observed for the first time in the subepicardium covering the dorsal side of the ventricle and atrioventricular canal at HH24.
The earliest myocardial invasion by endothelial precursor cells was observed from HH20 onward, although not in the region of this early vessel network, but in the inner curvature (Fig. 3). As was deduced from the position of these endothelial precursor cells in serial sections, they were derived from or connected to the endothelium of the more caudally located subepicardial capillaries. Because the QH1 antibody stains endocardial cells equally well, it appeared to be difficult to discern PEO-associated endothelial precursor cells in the myocardium from the endocardium-lined ends of the trabecular lumina.
To circumvent the difficulty that endocardial cells closely resemble endothelial (precursor) cells in an immunohistochemical QH1 staining, chimeric embryos were generated, in which the PEO of a quail embryo was transplanted to the pericardial cavity of a chicken embryo of equivalent age. In the resulting quail-chicken chimeras, QH1 could react only with the PEO-associated endothelial cells, leaving the chicken-derived endocardial cells unstained. A developmental series of quail-chicken chimeras (HH19–30) was made, in which the donor PEO was placed at several sites along the developing heart tube at HH16–17 (Fig. 1, Table 1), just at the time point when proepicardial cells start to transverse the pericardial cavity to reach the heart. The chicken host PEO was fully preserved. Thus, transplantaton did not interfere with the endogenous outgrowth of the chicken PEO and the heart could develop normally (Poelmann et al., 1993). This could also be deduced from the fact that the embryos had normal heart morphology.
With respect to the site of transplantation, we observed that PEO attachment to the heart and epicardial outgrowth succeeded in 31 out of 56 surviving embryos when the PEO was placed adjacent to the sinus venosus or in the inner curvature of the looping heart. In contrast, PEOs transplanted dorsocaudally of the ventricular myocardium either did not attach in 15 out of 25 cases or attached to the liver in the remaining 10 embryos. This may be indicative for a limited capacity of the ventricular myocardium to attract PEO-derived cells to its surface.
The site and extent of quail epicardial outgrowth in the embryos where the PEO had attached well have been summarized in Table 2. The presence of a subepicardial mesenchymal layer was a prerequisite for the ingrowth of PEO-derived cells into the underlying myocardium. More surprisingly, we observed that only the myocardium of the inner curvature was permissive for endothelial precursor cells and mesenchymal EPDCs between stage HH19 and HH22 (Fig. 4). The myocardium of the atrioventricular canal and that of the outflow tract were not invaded by PEO-derived cells, not even when a multicellular layer of subepicardial mesenchyme was present. The myocardium of the inner curvature is not covered with cushion tissue at its luminal side. However, PEO-derived cell invasion of the myocardium was not directly related to the absence of cushion tissue, since it was also observed in cushion-bearing myocardium of the atrioventricular canal at later stages of development (not shown). From HH23 onward, PEO-derived cells were also observed in the myocardium of the atria (Fig. 5A–C) and in the ventricular myocardium (Fig. 5D and E). The myocardium of the outflow tract was not permissive for EPDCs, at least untill HH26. Only at HH30 were EPDCs observed in the outflow tract myocardium up to its most distal border (Fig. 5H–J).
|Harvesting stage (HH)||SV||A||AVC||V||IC||OT|
From our set of quail-chicken chimeras, we could not precisely infer whether the endothelial precursor cells and EPDCs reached their intramyocardial locations only via the epicardium on the outside, or also by spreading within the myocardium. Only sporadically quail-derived EPDCs were observed in the myocardium underneath a layer of chicken-derived epicardium, and most quail EPDCs were deposited in the myocardium beneath a quail-derived QCPN-positive epicardial region. Figure 5D and E illustrate how an epicardium rich in quail cells delivers many quail EPDCs to the underlying myocardium, whereas an epicardium with less quail cells covers a myocardium in which less QCPN-positive EPDCs are present. Thus, endothelial precursor cell and EPDC migration from the epicardial to the myocardial layer seems to be a quite local process. Along the heart tube, the endothelial QH1 staining in the myocardium colocalized with the nuclear signal of the QCPN-positive quail cells in a large majority (95%) of the embryos, indicating that the entrance of endothelial precursor cells into the myocardium might precede that of mesenchymal EPDCs. EPDCs penetrating beyond the compact myocardium into the trabecular myocardium and subendocardial space were mostly found underneath those regions of the compact myocardium that were supplied well with endothelial (precursor) cells (Fig. 5F and G).
In the present study, we analyzed the behavior of early coronary contributors both in normal quail embryos and in proepicardial quail-chicken chimeras. Many aspects of the development of the coronary vasculature have been studied earlier by our group and others (Luttun and Carmeliet, 2003). For example, the intimate topographical relationship of liver primordium and the sinus venosus was documented by Virágh et al. (1993). The liver primordium is highly vascularized with developing capillaries, which, together with the perihepatic mesenchyme, enter the wall of the sinus venosus. It has been further observed that the caudal part of the splanchnic vessel plexus becomes part of the liver sinusoids, and that the more cranial part extends in the direction of the proepicardial organ (Vrancken Peeters et al., 1997). Thus, the endocardial cells that make up the capillaries developing in the proepicardial strands can be considered to be continuous with the endothelial cells of the liver sinusoids. Further evidence for a role of liver-derived endothelial (precursor) cells in the development of the coronary vasculature comes from earlier chimera experiments, in which the quail PEO was excised very precisely and transplanted to the pericardial cavity of a chicken host without any liver tissue. In the resulting embryos, quail-derived endothelial cells were never observed (Poelmann et al., 1993). Although the precise origin of the endothelial (precursor) cells in the PEO is still a matter of debate (Kattan et al., 2004), indirect proof of endothelial (precursor) cell migration through the PEO has been provided recently by the finding that larger numbers of QH1-positive cells were observed at the base of the PEO than in its villous tips in HH17 quail embryos (Kattan et al., 2004).
In earlier studies, true vascularization in the subepicardium, defined by the presence of lumenized capillaries, was described not to occur before HH25 (Poelmann et al., 1993; Virágh et al., 1993; Vrancken Peeters et al., 1997). We now show that lumenized endothelial vessels, in tissue sections sometimes observed with an erythrocyte inside, can be found in the subepicardium already at stage HH19. Because some of these were localized close to the developing liver, they may well have been arisen by sprouting from the sinus venosus-liver plexus. Thus, coronary formation at the dorsal side of the developing heart, at the attachment site of the outgrowing PEO, would be initiated by angiogenesis, rather than by vasculogenesis. On the other hand, as was described both in earlier and in more recent publications, large hemangioblastic cells were observed in the epicardium, the subepicardium, and on the naked heart surface (Vrancken Peeters et al., 1997; Kattan et al., 2004). These are indicative for the de novo vasculogenesis that has been shown to be instrumental in coronary development (Mikawa and Fischman, 1992).
In the young quail-chicken chimeras (HH19–23), only in the inner curvature were quail-derived EPDCs able to migrate further than the subepicardium and to find a permissive myocardium to migrate into. PEO transplants located next to the outflow tract or close to the developing ventricle failed to deposit coronary precursor cells into the myocardial layer of the AV canal, the atria, and the ventricles until HH23/24. To our knowledge, this is the first in vivo study demonstrating that the invasion of coronary contributors into the myocardium and thus the onset of myocardial vascularization depend on characteristics of the underlying myocardium. In the avian embryo, the PEO protrudes from the dorsal mesothelium to the dorsal side of the developing common ventricle, from where it begins to ensheath the myocardium in the region of the atrioventricular canal. Instead of entering the myocardium in this region, the PEO-associated endothelial cells travel all the way to the inner curvature to initiate the formation of intramyocardial coronary capillaries. In our quail-chicken chimera experiments, quail endothelial precursor cell and EPDC ingrowth started exclusively in the myocardium of the inner curvature, whereas outflow tract myocardium was refractory to this, even when the quail PEO was attached directly onto it. This also confirms the notion that the myocardial microenvironment is an important determinant for endothelial precursor cell and EPDC migration and coronary vessel formation (Morabito et al., 2001, 2002). As for the nature of the myocardial signals, we can only speculate. A marked anatomical difference between the myocardium of the inner curvature compared with that of the atrioventricular canal and the outflow tract is that there is no endocardial cushion present on the inside. Possibly, angiogenic regulators (inhibitors) secreted by the endocardium or cushion mesenchyme induce the myocardium to be nonpermissive for endothelial cells coming from the other side in the AV canal and outflow tract. Good candidates to function as such antiangiogenic regulators are TGF-β2 and TGF-β3. In vitro analysis showed that TGF-β1, -2, and -3 inhibit the myocardial signals that regulate epicardial EMT (Morabito et al., 2001). In chicken embryos, both TGF-β2 and -3 are expressed in the myocardium and endocardium of the AV canal and outflow tract during cushion formation around stage HH17 (Boyer et al., 1999), just preceding the endothelial (precursor) ingrowth of the inner curvature. In mouse embryos, strong TGF-β2 expression is seen in the outflow tract and atrioventricular cushions and underlying myocardium between embryonic day (ED) 9.5 and ED12.5, comparable to chicken stage HH20–25. At ED11.5–12.5, epicardial TGF-β2 and -3 expression increases, especially in the epicardium covering the ventral side of the developing ventricles (Molin et al., 2003). This might explain why the subepicardium is relatively thin at this location. After ED12.5, TGF-β2 expression remains high in the cushion mesenchyme of the outflow tract and AV canal. On the proangiogenic side, VEGF becomes upregulated in the atrioventricular field of the heart from ED10.5 onward (Dor et al., 2001). VEGF expression stimulates endothelial proliferation and migration (Carmeliet, 2000) and has been shown to activate the endothelial proteolytic machinery (Prager et al., 2004). Thus, the AV region might become prone to invasion by epicardially derived endothelial and mesenchymal cells, whereas the outflow tract myocardium remains inaccessible until the VEGF signaling routes can be fully employed. In quail embryos, increased endothelial VEGF-R2 and VEGF-R3 expression coincides with the ingrowth of coronary capillaries at the base of the truncus arteriosus to form the coronary plexus that precedes the formation of the coronary stems (Tomanek et al., 2002).
The fact that we did not find myocardial endothelial (precursor) cells and EPDCs of quail origin in regions covered by a chicken epicardium indicates that myocardial entry of these cells is indeed a very local process, as was already suggested by earlier findings in older chimeric embryos (Gittenberger-de Groot et al., 1998). The local delivery of coronary components only to the underlying myocardium could also be seen in stage HH35 quail embryos in which epicardial outgrowth was partially inhibited. Only the ventricular regions covered with epicardium acquired a compact myocardium with regularly arranged coronary vessels. Naked compact ventricular myocardium was completely devoid of these vessels, indicating that the local presence of coronary precursor cells is a prerequisite for coronary vessel formation (data not shown).
In the set chimeras that we analyzed, the endothelial (precursor) cells seemed to be the first to enter the myocardium. At later stages, more intramyocardial mesenchymal EPDCs were observed. Only in the ventricular myocardium, where epicardium-derived fibroblasts also contribute to myocardial rigidity, did the EPDCs outnumber the endothelial (precursor) cells from stage HH25/26 onward. However, in myocardial regions where coronary artery stabilization is one of the EPDC functions, many of the proepicardium-derived cells are endothelial cells, which seem to be followed by the mesenchymal EPDCs. It is tempting to speculate that, probably via PDGF-B signaling (Nishishita and Lin, 2004), the endothelial (precursor) cells pave the way for the entrance of the mesenchymal cells that will assist in the formation of stable vessels. Further support for the idea that penetration by endothelial (precursor) cells may facilitate mesenchymal EPDC migration comes from our observation in older chimeras that only in regions where the compact myocardium was supplied with numerous quail endothelial cells did the underlying trabecular myocardium contain many quail-derived EPDCs.
Finally, between HH20 and HH24, the inner curvature is not only the site where coronary formation starts, it is also a region that is very much involved in the last phase of the cardiac looping process. During this phase, the proximal two-thirds of the outflow tract or primitive conus shifts from the right lateral position to its final position, ventral to the right atrium (Männer, 2000). In quail embryos in which proepicardial outgrowth was inhibited, cardiac looping was severely disturbed (Gittenberger-de Groot et al., 2000). Although we did not directly address the issue, the present study might give a first concrete clue for a role of the epicardium in the looping process. We hypothesize that the selective ingrowth of proepicardially derived endothelial cells into the inner curvature is the starting signal for the myocardium to rearrange its fibrils and for the conus to shift leftward.
The authors gratefully acknowledge Jan H. Lens and Bas Blankevoort for excellent photographic assistance and digital artwork. They also thank Jaime Martodihardjo for the initial characterization of the chimeric embryos and Daniël G.M. Molin for critical reading of the manuscript.
- 1999. TGFbeta2 and TGFbeta3 have separate and sequential activities during epithelial-mesenchymal cell transformation in the embryonic heart. Dev Biol 208: 530–545. , , , , , .
- 2000. Mechanisms of angiogenesis and arteriogenesis. Nat Med 6: 389–395. .
- 2002. The hemangioblast: a common progenitor of hematopoietic and endothelial cells. J Hematother Stem Cell Res 11: 91–101. .
- 2001. A novel role for VEGF in endocardial cushion formation and its potential contribution to congenital heart defects. Development 128: 1531–1538. , , , , , , .
- 1998. Epicardial derived cells, EPDCs, contribute a novel population to the myocardial wall and the atrioventricular cushions. Circ Res 82: 1043–1052. , , , , .
- 2000. Epicardial outgrowth inhibition leads to compensatory mesothelial outflow tract collar and abnormal cardiac septation and coronary formation. Circ Res 87: 969–971. , , , , .
- 1951. A series of normal stages in the development of the chick embryo. J Morphol 88: 49–92. , .
- 2004. Formation and remodeling of the coronary vascular bed in the embryonic avian heart. Dev Dyn 230: 34–43. , , .
- 1995. Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development 121: 489–503. , , , , , , .
- 2003. Ets-1 and Ets-2 transcription factors are essential for normal coronary and myocardial development in chicken embryos. Circ Res 92: 749–756. , , , , , , .
- 2003. De novo vasculogenesis in the heart. Cardiovasc Res 58: 378–389. , .
- 1992. The development of pericardial villi in the chick embryo. Anat Embryol 186: 379–385. .
- 2000. Cardiac looping in the chick embryo: a morphological review with special reference to terminological and biomechanical aspects of the looping process. Anat Rec 259: 248–262. .
- 1992. Retroviral analysis of cardiac morphogenesis: discontinuous formation of coronary vessels. Proc Natl Acad Sci USA 89: 9504–9508. , .
- 2003. Expression patterns of Tgfbeta1-3 associate with myocardialisation of the outflow tract and the development of the epicardium and the fibrous heart skeleton. Dev Dyn 227: 431–444. , , , , , , , .
- 1999. Yac complementation shows a requirement for Wt1 in the development of epicardium, adrenal gland and throughout nephrogenesis. Development 126: 1845–1857. , , , , .
- 2001. Positive and negative regulation of epicardial-mesenchymal transformation during avian heart development. Dev Biol 234: 204–215. , , , , .
- 2002. Mechanisms of embryonic coronary artery development. Curr Opin Cardiol 17: 235–241. , , .
- 2003. Evidence for an extracellular matrix bridge guiding proepicardial cell migration to the myocardium of chick embryos. Dev Dyn 227: 511–523. , , .
- 2004. Angiopoietin 1, PDGF-B, and TGF-beta gene regulation in endothelial cell and smooth muscle cell interaction. J Cell Biochem 91: 584–593. , .
- 1993. Development of the cardiac coronary vascular endothelium, studied with antiendothelial antibodies, in chicken-quail chimeras. Circ Res 73: 559–568. , , , , .
- 2004. Vascular endothelial growth factor (VEGF) induces rapid prourokinase (pro-uPA) activation on the surface of endothelial cells. Blood 103: 955–962. , , , , .
- 2000. FOG-2, a cofactor for GATA transcription factors, is essential for heart morphogenesis and development of coronary vessels from epicardium. Cell 101: 729–739. , , , , , , , .
- 2002. Role of VEGF family members and receptors in coronary vessel formation. Dev Dyn 225: 233–240. , , , , .
- 1993. Early development of quail heart epicardium and associated vascular and glandular structures. Anat Embryol 188: 381–393. , , , .
- 1995. Cytokeratins as a marker for epicardial formation in the quail embryo. Anat Embryol 191: 503–508. , , , .
- 1997. The development of the coronary vessels and their differentiation into arteries and veins in the embryonic quail heart. Dev Dyn 208: 338–348. , , , , , .
- 1999. Smooth muscle cells and fibroblasts of the coronary arteries derive from epithelial-mesenchymal transformation of the epicardium. Anat Embryol 199: 367–378. , , , .
- 2004. The epicardium and epicardially derived cells (EPDCs) as cardiac stem cells. Anat Rec 276A: 43–57. , .
- 1993. Development of the liver in the chicken embryo: II, erythropoietic and granulopoietic cells. Anat Rec 235: 131–143. , .
- 1995. Cell adhesion events mediated by α4 integrins are essential in placental and cardiac development. Development 121: 549–560. , , .