Design-based stereological analysis of the lung parenchymal architecture and alveolar type II cells in surfactant protein A and D double deficient mice†
Article first published online: 7 AUG 2005
Copyright © 2005 Wiley-Liss, Inc.
The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume 286A, Issue 2, pages 885–890, October 2005
How to Cite
Jung, A., Allen, L., Nyengaard, J. R., Gundersen, H. J. G., Richter, J., Hawgood, S. and Ochs, M. (2005), Design-based stereological analysis of the lung parenchymal architecture and alveolar type II cells in surfactant protein A and D double deficient mice. Anat. Rec., 286A: 885–890. doi: 10.1002/ar.a.20225
Presented in part at the Anatomical Congress in Würzburg, Germany, 2002, and the European Respiratory Society (ERS) Congress in Vienna, Austria, 2003.
- Issue published online: 21 SEP 2005
- Article first published online: 7 AUG 2005
- Manuscript Accepted: 6 JUL 2005
- Manuscript Received: 2 NOV 2004
- Medical Faculty of the University of Göttingen. Grant Number: 1400490
- Alexander von Humboldt Foundation Feodor Lynen Fellowship
- National Institutes of Health. Grant Numbers: HL-24075, HL-58047
- type II cells;
- lamellar bodies;
Alveolar epithelial type II cells synthesize and secrete surfactant. The surfactant-associated proteins A and D (SP-A and SP-D), members of the collectin protein family, participate in pulmonary immune defense, modulation of inflammation, and surfactant metabolism. Both proteins are known to have overlapping as well as distinct functions. The present study provides a design-based stereological analysis of adult mice deficient in both SP-A and SP-D (A−D−) with special emphasis on parameters characterizing alveolar architecture and surfactant-producing type II cells. Compared to wild-type, A−D− mice have fewer and larger alveoli, an increase in the number and size of type II cells, as well as more numerous and larger alveolar macrophages. More surfactant-storing lamellar bodies are seen in type II cells, leading to a threefold increase in the total volume of lamellar bodies per lung, but the mean volume of a single lamellar body remains constant. These results demonstrate that chronic deficiency of SP-A and SP-D in mice leads to parenchymal remodeling, type II cell hyperplasia and hypertrophy, and disturbed intracellular surfactant metabolism. The design-based stereological approach presented here provides a framework for the quantitative lung structure analysis in gene-manipulated mice as well as in human lung disease. © 2005 Wiley-Liss, Inc.