Stereology and ultrastructure of the salivary glands of diabetic Nod mice submitted to long-term insulin treatment
Version of Record online: 2 SEP 2005
Copyright © 2005 Wiley-Liss, Inc.
The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume 286A, Issue 2, pages 930–937, October 2005
How to Cite
Caldeira, E. J., Camilli, J. A. and Cagnon, V. H. A. (2005), Stereology and ultrastructure of the salivary glands of diabetic Nod mice submitted to long-term insulin treatment. Anat. Rec., 286A: 930–937. doi: 10.1002/ar.a.20236
- Issue online: 21 SEP 2005
- Version of Record online: 2 SEP 2005
- Manuscript Accepted: 30 JUN 2005
- Manuscript Received: 4 MAY 2005
- Foundation of Support to the Research of the State of São Paolo (FAPESP). Grant Number: 03/1377-3
- salivary gland;
- autoimmune diabetes;
- insulin treatment
Insulin-dependent diabetes mellitus compromises the salivary glands, altering their morphology and the mechanisms of salivation, which are fundamental for oral health. Thus, the aim of the present study was to determine the effects of prolonged insulin treatment on the morphology of the salivary glands in Nod mice. Forty-five female mice were divided into five groups: nine positive diabetic Nod mice for 10 days (group 1), nine positive diabetic Nod mice for 20 days (group 2), nine diabetic Nod mice for 10 days (group 3), nine diabetic Nod mice for 20 days (group 4), and nine nondiabetic BALB/c mice (group 5). Animals of groups 3 and 4 received 4–5 U of insulin daily, whereas animals of groups 1, 2, and 5 received the same dose of physiological saline simulating the experimental conditions. Samples of the salivary glands were analyzed by light, transmission, and scanning electron microscopies. The results showed intense alterations in diabetic animals characterized by nuclear and cytoplasmic atrophy, biomembrane disorganization, an increase in fibrillar components of the extracellular matrix, and the presence of inflammatory cells. Insulin treatment exerted positive effects on the recovery of the changes resulting from the diabetic state in both parotid and submandibular glands but the pattern continued to be altered. It can be concluded that, in addition to compromising the processes of tissue maintenance and renewal, tissue destructuring leads to alterations in functional mechanisms in both diabetic animals and animals submitted to glycemic control. © 2005 Wiley-Liss, Inc.