Cloning and expression of gallerimycin, an antifungal peptide expressed in immune response of greater wax moth larvae, Galleria mellonella
Article first published online: 11 JUN 2003
Copyright © 2003 Wiley-Liss, Inc.
Archives of Insect Biochemistry and Physiology
Volume 53, Issue 3, pages 125–133, July 2003
How to Cite
Schuhmann, B., Seitz, V., Vilcinskas, A. and Podsiadlowski, L. (2003), Cloning and expression of gallerimycin, an antifungal peptide expressed in immune response of greater wax moth larvae, Galleria mellonella. Arch. Insect Biochem. Physiol., 53: 125–133. doi: 10.1002/arch.10091
- Issue published online: 11 JUN 2003
- Article first published online: 11 JUN 2003
- Manuscript Accepted: 31 MAR 2003
- Manuscript Received: 4 OCT 2002
- Deutsche Forschungsgemeinschaft (DFG). Grant Number: Go 165/14-4
- insect immunity;
- antifungal peptide;
A novel defensin-like peptide was identified in the greater wax moth, Galleria mellonella. It was discovered in a haemocyte cDNA bank enriched with transcripts upregulated after immune challenge via subtractive hybridisation and suppressive PCR. The deduced amino acid sequence of the defensin-like peptide exhibits similarities to the antifungal peptides drosomycin from Drosophila melanogaster and heliomicin from Heliothis virescens. Therefore, it has been termed gallerimycin. Upregulation of gallerimycin after stimulation of the immune system by LPS-injection was demonstrated by quantitative real-time PCR. A full-size cDNA was cloned and overexpressed in Escherichia coli Origami cells in order to obtain a functional peptide with disulfide bridges. The recombinant peptide was active against the entomopathogenic fungus Metarhizium anisopliae, but not against yeast, gram-negative and gram-positive bacteria. Arch. Insect Biochem. Physiol. 53:125–133, 2003. © 2003 Wiley-Liss, Inc.