Male accessory gland derived factors can stimulate oogenesis and enhance oviposition in Helicoverpa armigera (Lepidoptera: Noctuidae)

Authors

  • Zhao-Yang Jin,

    1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Academia Sinica, Beijing, China
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  • He Gong

    Corresponding author
    1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Academia Sinica, Beijing, China
    • State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Academia Sinica, Beijing 100080, China
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Abstract

In Helicoverpa armigera, female moths began to lay eggs on the third day after emergence. Mating stimulated earlier egg maturation/oogenesis (P = 0.002) and oviposition (P << 0.01). We established a suitable bioassay model for the influence of male accessory glands (MAG) on the physiology of virgin females: Crude extracts of MAG (2- to 3-day-old) were injected into 2-day-old virgin females, and the injected females were dissected 20 h after mating. It was shown that crude extracts of MAG stimulated earlier egg maturation (P < 0.001) and oviposition (the oviposition ratio was more than 2 times the ratio of the control). Proteinaceous components in crude extracts purified by fractionation and sub-fractionation in reverse phase high performance liquid chromatography also stimulated earlier egg maturation (P < 0.01) and ovipositon (more than 2 times the ratio of the control), and we called them the oogenesis and ovipostion factors (OOSF). With SDS-PAGE, the molecular mass of the bands from OOSF was estimated to be between 55–66 KD. Arch. Insect Biochem. Physiol. 46:175–185, 2001. © 2001 Wiley-Liss, Inc.

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