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Adenanthera pavonina trypsin inhibitor retard growth of Anagasta kuehniella (Lepidoptera: Pyralidae)

Authors

  • Maria Lígia Rodrigues Macedo,

    Corresponding author
    1. Laboratório de Purificação de Proteínas e suas Funções Biológicas, Depto. De Tecnologia de Alimentos e Saúde Pública, CCBS, Universidade Federal de Mato Grosso do Sul, CP 549, CEP 79070-900
    • Departamento de Tecnologia de Alimentos e Saúde Pública, Centro de Ciências Biológicas e da Saúde, Universidade Federal do Mato Grosso do Sul (UFMS), Cidade Universitária S/N - Caixa Postal 549, CEP 79070-900 - Campo Grande – MS, Brazil
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  • Roberta Aparecida Durigan,

    1. Laboratório de Purificação de Proteínas e suas Funções Biológicas, Depto. De Tecnologia de Alimentos e Saúde Pública, CCBS, Universidade Federal de Mato Grosso do Sul, CP 549, CEP 79070-900
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  • Desiree Soares da Silva,

    1. Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP 13093-970, Campinas, SP, Brazil
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  • Sérgio Marangoni,

    1. Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP 13093-970, Campinas, SP, Brazil
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  • Maria das Graças Machado Freire,

    1. ISE-CENSA – CEP: 28035-310, Campos dos Goytacazes - Rio de Janeiro – Brazil
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  • José Roberto Postali Parra

    1. Departamento de Entomologia, Fitopatologia e Zoologia Agrícola. Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba, SP, Brazil
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Abstract

Anagasta kuehniella is a polyphagous pest that feeds on a wide variety of stored products. The possible roles suggested for seed proteinase inhibitors include the function as a part of the plant defensive system against pest via inhibition of their proteolytic enzymes. In this study, a trypsin inhibitor (ApTI) was purified from Adenanthera pavonina seed and was tested for insect growth regulatory effect. The chronic ingestion of ApTI did result in a significant reduction in larval survival and weight. Larval and pupal developmental time of larvae fed on ApTI diet at 1% was significantly longer; the larval period was extended by 5 days and pupal period was 10 days longer, therefore delaying by up to 20 days and resulting in a prolonged period of development from larva to adult. As a result, the ApTI diet emergence rate was only 28% while the emergence rate of control larvae was 80%. The percentage of surviving adults (%S) decreased to 62%. The fourth instar larvae reared on a diet containing 1% ApTI showed a decrease in tryptic activity of gut and that no novel proteolytic form resistant to ApTI was induced. In addition, the tryptic activity in ApTI -fed larvae was sensitive to ApTI. These results suggest that ApTI have a potential antimetabolic effect when ingested by A. kuehniella. © 2010 Wiley Periodicals, Inc.

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