The authors declare that they have no conflict of interest. This article reports results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation by the USDA for its use.
Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination†
Article first published online: 22 JUN 2010
This article is a US government work and, as such, is in the public domain in the United States of America. Published 2010 Wiley Periodicals, Inc.
Archives of Insect Biochemistry and Physiology
Volume 74, Issue 3, pages 179–204, July 2010
How to Cite
Olafson, P. U., Lohmeyer, K. H. and Dowd, S. E. (2010), Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination. Arch. Insect Biochem. Physiol., 74: 179–204. doi: 10.1002/arch.20372
- Issue published online: 22 JUN 2010
- Article first published online: 22 JUN 2010
- Stomoxys calcitrans;
- stable fly;
- sex determination;
- reproductive behavior
The stable fly, Stomoxys calcitrans L. (Diptera: Muscidae), is one of the most significant pests of livestock in the United States. The identification of targets for the development of novel control for this pest species, focusing on those molecules that play a role in successful feeding and reproduction, is critical to mitigating its impact on confined and rangeland livestock. A database was developed representing genes expressed at the immature and adult life stages of the stable fly, comprising data obtained from pyrosequencing both immature and adult stages and from small-scale sequencing of an antennal/maxillary palp–expressed sequence tag library. The full-length sequence and expression of 21 transcripts that may have a role in chemosensation is presented, including 13 odorant-binding proteins, 6 chemosensory proteins, and 2 odorant receptors. Transcripts with potential roles in sex determination and reproductive behaviors are identified, including evidence for the sex-specific expression of stable fly doublesex- and transformer-like transcripts. The current database will be a valuable tool for target identification and for comparative studies with other Diptera. Published 2010 Wiley Periodicals, Inc.†