SUPPRESSION OF AcMNPV REPLICATION BY ADF AND THYMOSIN PROTEIN UP-REGULATION IN A NEW TESTIS CELL LINE, Ha-shl-t

Authors

  • Xiaoqian Zhang,

    1. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China
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  • Ming Chen,

    1. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China
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  • Xinlei Ma,

    1. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China
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  • Xiaofan Zhao,

    1. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China
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  • Jinxing Wang,

    1. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China
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  • Honglian Shao,

    Corresponding author
    • Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China
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  • Qisheng Song,

    1. Division of Plant Sciences, University of Missouri, Columbia, Missouri, USA
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  • David Stanley

    1. Division of Plant Sciences, University of Missouri, Columbia, Missouri, USA and U.S. Department of Agriculture, Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, Missouri, USA
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  • Grant sponsor: National Natural Science Foundation of China; Grant numbers: 30770281; 31071977; Grant sponsor: Shandong Provincial Natural Science Foundation, China; Grant number: Y2007D51.

Correspondence to: Honglian Shao, School of Life Sciences, Shandong University, Jinan 250100, China. E-mail: shaohl@sdu.edu.cn

Abstract

Host cytoskeletons facilitate the entry, replication, and egress of viruses because cytoskeletons are essential for viral survival. One mechanism of resisting viral infections involves regulating cytoskeletal polymerization/depolymerization. However, the molecular mechanisms of regulating these changes in cytoskeleton to suppress viral replication remain unclear. We established a cell line (named Ha-shl-t) from the pupal testis of Helicoverpa armigera (Lepidoptera: Noctuidae). The new testis cell line suppresses Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV) replication via disassembly of cytoskeleton. Up-regulation of thymosin (actin disassembling factor) and adf (actin depolymerizing factor) reduces F-actin. Silencing thymosin or adf or treating cells with the F-actin stabilizer phalloidin led to increased AcMNPV replication, while treating cells with an F-actin assembly inhibitor cytochalasin B decreased viral replication. We infer that Ha-shl-t cells utilize F-actin depolymerization to suppress AcMNPV replication by up-regulating thymosin and adf. We propose Ha-shl-t as a model system for investigating cytoskeletal regulation in antiviral action and testicular biology generally.

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