Involvement of CD4+,CD57+ T cells in the disease activity of rheumatoid arthritis
Article first published online: 5 FEB 2002
Copyright © 2002 by the American College of Rheumatology
Arthritis & Rheumatism
Volume 46, Issue 2, pages 379–384, February 2002
How to Cite
Maeda, T., Yamada, H., Nagamine, R., Shuto, T., Nakashima, Y., Hirata, G. and Iwamoto, Y. (2002), Involvement of CD4+,CD57+ T cells in the disease activity of rheumatoid arthritis. Arthritis & Rheumatism, 46: 379–384. doi: 10.1002/art.10133
- Issue published online: 5 FEB 2002
- Article first published online: 5 FEB 2002
- Manuscript Accepted: 24 SEP 2001
- Manuscript Received: 19 JUL 2001
To evaluate the relationship between the frequency of peripheral CD57+ T cells and the physical status of rheumatoid arthritis (RA) patients, and to perform cytokine analysis of these CD57+ T cells.
Four-color fluorescence-activated cell sorter analysis was performed to detect both cell surface antigens and intracellular cytokines in peripheral blood leukocytes, using monoclonal antibodies against CD3, CD4, CD8, CD57, interferon-γ (IFNγ), and interleukin-4 (IL-4). RA patients were clinically evaluated with a modified Health Assessment Questionnaire (M-HAQ), joint score, face scale, and visual analog scale (VAS) assessing pain and disease activity.
There was a significant correlation between the frequency of CD4+,CD57+ T cells and erythrocyte sedimentation rate (ESR), whereas a correlation was not found between the frequency of CD8+,CD57+ T cells and ESR. The frequency of CD4+,CD57+ T cells also showed a significant correlation with the mHAQ score, VAS, and face scale. Again, there was no significant correlation between the above-mentioned clinical scores and the frequency of CD8+,CD57+ T cells. Flow cytometric analysis of intracellular cytokines revealed that 14.5% of the CD57+ T cells produced IFNγ, whereas only 2.8% of the CD57+ T cells produced IL-4 in RA patients.
Evidence showing that the frequency of CD4+,CD57+ T cells among CD3+ cells of RA patients had a significant correlation not only with ESR but also with the physical status of the patients, and that a large proportion of the CD4+,CD57+ T cells had the capacity to produce IFNγ, strongly suggests that these CD4+,CD57+ T cells are involved in the immunopathogenesis of RA.