Expression of osteopontin at sites of bone erosion in a murine experimental arthritis model of collagen-induced arthritis: Possible involvement of osteopontin in bone destruction in arthritis
Article first published online: 5 APR 2002
Copyright © 2002 by the American College of Rheumatology
Arthritis & Rheumatism
Volume 46, Issue 4, pages 1094–1101, April 2002
How to Cite
Ohshima, S., Kobayashi, H., Yamaguchi, N., Nishioka, K., Umeshita-Sasai, M., Mima, T., Nomura, S., Kon, S., Inobe, M., Uede, T. and Saeki, Y. (2002), Expression of osteopontin at sites of bone erosion in a murine experimental arthritis model of collagen-induced arthritis: Possible involvement of osteopontin in bone destruction in arthritis. Arthritis & Rheumatism, 46: 1094–1101. doi: 10.1002/art.10143
- Issue published online: 5 APR 2002
- Article first published online: 5 APR 2002
- Manuscript Accepted: 2 NOV 2001
- Manuscript Received: 21 MAR 2001
- Ministry of Education, Science, and Culture
- Ministry of Health and Welfare of Japan
- Kowa Pharmaceutical Company
- Ono Pharmaceutical Company
To investigate the involvement of osteopontin (OPN) in bone destruction in a murine experimental arthritis model of collagen-induced arthritis (CIA).
The expression of OPN was examined at both the messenger RNA (mRNA) and protein levels in various arthritic lesions in mice with CIA by in situ hybridization and immunohistochemistry, respectively. In addition, the expression of αvβ3 integrin, a receptor for OPN, the ligation of which is thought to be essential for bone resorption by osteoclasts, was examined by immunohistochemistry. Plasma concentrations of OPN were measured at different time points in the course of CIA by enzyme-linked immunosorbent assay.
OPN mRNA was detected mainly at sites of bone erosion in arthritic lesions, where activated osteoclasts were present; OPN protein was also detected at sites of bone erosion. In the arthritic synovium, OPN was predominantly expressed in the synovial lining layer, but not in lymphoid aggregates. In addition, αvβ3 integrin was detected coincident with OPN at sites of bone erosion (bone–pannus junction). Plasma OPN levels were markedly elevated at the time points that corresponded to arthritis flares, and higher levels were maintained during the progression of arthritis.
OPN may mediate bone resorption by osteoclasts in arthritis through ligation with its receptor, αvβ3 integrin. OPN may be a useful therapeutic target molecule in the prevention of bone destruction in arthritis.