Acidic cysteine endoproteinase cathepsin K in the degeneration of the superficial articular hyaline cartilage in osteoarthritis
Article first published online: 5 APR 2002
Copyright © 2002 by the American College of Rheumatology
Arthritis & Rheumatism
Volume 46, Issue 4, pages 953–960, April 2002
How to Cite
Konttinen, Y. T., Mandelin, J., Li, T.-F., Salo, J., Lassus, J., Liljeström, M., Hukkanen, M., Takagi, M., Virtanen, I. and Santavirta, S. (2002), Acidic cysteine endoproteinase cathepsin K in the degeneration of the superficial articular hyaline cartilage in osteoarthritis. Arthritis & Rheumatism, 46: 953–960. doi: 10.1002/art.10185
- Issue published online: 5 APR 2002
- Article first published online: 5 APR 2002
- Manuscript Accepted: 26 NOV 2001
- Manuscript Received: 10 APR 2001
- clinical EVO research grants. Grant Numbers: TYH 0056, TYH 0215, TYH 0341, TYH 8307
- Invalid Foundation. Grant Number: 9750/2
- Academy of Finland/Centre for Technological Advancement (TEKES)/Ministry of Education (OPM)/University of Helsinki Group of Excellence scheme
To measure cartilage pH in patients with osteoarthritis (OA) and to analyze the presence of cathepsin K, the recently discovered acidic endoproteinase, in phenotypically altered chondrocytes.
Intraoperative measurements of the pH of clinically normal, fibrillated, superficially fissured, and deeply fissured cartilage surfaces (grades 0–3, respectively) in OA patients undergoing primary hip replacement surgery were performed with the use of a sting electrode sterilized with microbicidic plasma. Fluorescent pH probes were used for in situ assessment of cartilage matrix pH. Cathepsin K was assessed using quantitative reverse transcriptase–polymerase chain reaction and immunohistochemistry methods.
The pH of grade 0 cartilage surfaces was 7.1 ± 0.4 (mean ± SD), compared with 6.2 ± 0.9 (P < 0.05), 5.7 ± 1.0 (P < 0.001), and 5.5 ± 1.0 (P < 0.001) for grades 1–3 cartilage surfaces, respectively. Fluorescent pH probes and acid-dependent autocatalytic conversion of cathepsin K into its active, low molecular weight form in cartilage confirmed these findings. Cathepsin K messenger RNA levels increased in relation to the severity of OA, and the number of cathepsin K–containing chondrocytes increased from a mean ± SD of 12 ± 3 in grade 0 cartilage surfaces to 47 ± 7, 50 ± 6, and 100 ± 12 in grades 1–3 cartilage surfaces, respectively (P < 0.001 for all comparisons).
Acid-activated, but pharmacologically inhibitable, cathepsin K is induced in phenotypically altered chondrocytes in OA. The findings suggest that cathepsin K, rather than neutral matrix metalloproteinases, degrades the superficial gliding surfaces of the articular hyaline cartilage in OA.