Antibodies to vimentin intermediate filaments in sera from patients with systemic lupus erythematosus

Authors

  • Andrés Alcover PhD,

    Research Fellow, Corresponding author
    1. Centro de Biología Molecular
    2. Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Canto Blanco, Madrid-34, Spain; the Instituto de Enzimologia, Facultad de Medicina, Universidad Autonoma, Madrid, Spain; and the C. S. La Paz, Madrid, Spain.
    • Centro de Biologia Molecular, Universidad Autonoma, Canto Blanco, Madrid-34, Spain
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  • Jesús Molano MD,

    Staff
    1. Servicio Bioquímcia, C. S. La Paz
    2. Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Canto Blanco, Madrid-34, Spain; the Instituto de Enzimologia, Facultad de Medicina, Universidad Autonoma, Madrid, Spain; and the C. S. La Paz, Madrid, Spain.
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  • Jaime Renart PhD,

    Research Staff
    1. Institute de Enzimología
    2. Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Canto Blanco, Madrid-34, Spain; the Instituto de Enzimologia, Facultad de Medicina, Universidad Autonoma, Madrid, Spain; and the C. S. La Paz, Madrid, Spain.
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  • Antonio Gil-Aguado MD,

    Staff
    1. Servicio Medicina Interna, C. S. La Paz
    2. Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Canto Blanco, Madrid-34, Spain; the Instituto de Enzimologia, Facultad de Medicina, Universidad Autonoma, Madrid, Spain; and the C. S. La Paz, Madrid, Spain.
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  • Antonio Nieto MD, PhD,

    Research Staff
    1. Centro de Biología Molecular
    2. Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Canto Blanco, Madrid-34, Spain; the Instituto de Enzimologia, Facultad de Medicina, Universidad Autonoma, Madrid, Spain; and the C. S. La Paz, Madrid, Spain.
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  • Jesús Avila PhD

    Research Staff
    1. Centro de Biología Molecular
    2. Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Canto Blanco, Madrid-34, Spain; the Instituto de Enzimologia, Facultad de Medicina, Universidad Autonoma, Madrid, Spain; and the C. S. La Paz, Madrid, Spain.
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Abstract

Sera from patients with systemic lupus erythe-matosus and from healthy subjects were tested, using immunofluorescence and blotting techniques, for the presence of antibodies to intermediate filaments of the cytoskeleton of human skin fibroblasts. Both techniques showed that antibodies to intermediate filaments were found in a higher proportion of sera from patients (53%) than healthy subjects (9%). The antigen target was found to be the protein band that corresponded to vimentin (Mr 57,000).

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