Department of Immunology, Hospital de la Santa Creu i Sant Pau, Padre Claret, Barcelona, Spain.
Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts
Article first published online: 29 NOV 2005
Copyright © 1985 American College of Rheumatology
Arthritis & Rheumatism
Volume 28, Issue 12, pages 1356–1361, December 1985
How to Cite
Forman, M. S., Nakamura, M., Mimori, T., Gelpi, C. and Hardin, J. A. (1985), Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts. Arthritis & Rheumatism, 28: 1356–1361. doi: 10.1002/art.1780281207
- Issue published online: 29 NOV 2005
- Article first published online: 29 NOV 2005
- Manuscript Accepted: 28 MAY 1985
- Manuscript Received: 24 SEP 1984
- U.S. Public Health Service. Grant Numbers: AM-32549, AM-10493, AM-07107
RNA molecules immunoprecipitated with sera from patients who have rheumatic diseases can be readily detected in polyacrylamide gels by using ethidium bromide and silver stains. With these stains, we found that RNA patterns characteristic of a broad range of specific small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins were recognizable. The stains correctly identified antibodies to ribonucleoproteins in 33 (92%) of 36 patient sera selected for study because of known antibody specificities. The silver stain method detected antibodies to ribonucleoproteins in 25 (76%) of 33 patients with classic systemic lupus erythematosus, a frequency that approximated the frequency observed in the Lerner-Steitz assay, which is based on autoradiography. This approach considerably simplifies the latter radioimmunoassay with minimal loss of precision and sensitivity.