Characterization and functional studies of rheumatoid synovial mast cells: Activation by secretagogues, anti-IgE, and a histamine-releasing lymphokine

Authors

  • Dr. Barry Gruber MD,

    Corresponding author
    1. Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Northport Veterans Administration Hospital, Department of Pediatrics, State University of New York at Stony Brook, Health Sciences Center, Stony Brook, New York
    • Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, SUNY-Stony Brook, Stony Brook, NY 11794-8161
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  • Mark Poznansky MD,

    1. Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Northport Veterans Administration Hospital, Department of Pediatrics, State University of New York at Stony Brook, Health Sciences Center, Stony Brook, New York
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  • Eleonore Boss HTL, ASCP,

    1. Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Northport Veterans Administration Hospital, Department of Pediatrics, State University of New York at Stony Brook, Health Sciences Center, Stony Brook, New York
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  • Jacqueline Partin MS,

    1. Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Northport Veterans Administration Hospital, Department of Pediatrics, State University of New York at Stony Brook, Health Sciences Center, Stony Brook, New York
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  • Peter Gorevic MD,

    1. Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Northport Veterans Administration Hospital, Department of Pediatrics, State University of New York at Stony Brook, Health Sciences Center, Stony Brook, New York
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  • Allen P. Kaplan MD

    1. Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Northport Veterans Administration Hospital, Department of Pediatrics, State University of New York at Stony Brook, Health Sciences Center, Stony Brook, New York
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Abstract

Microscopic analysis of synovial specimens from 35 patients with rheumatoid arthritis (RA) and 7 patients with osteoarthritis revealed mast cell hyperplasia in perivascular regions, in fibrous interstitial areas, and clustered around the periphery of lymphoid aggregates. Metachromatic staining, immunofluorescence studies, and ultrastructural analysis revealed a single population of connective tissue-type mast cells with surface IgE receptors. Total extractable histamine of synovial tissue was 4.15 ± 2.30 μg/gm (n = 8) for RA synovium and 0.53 ± 0.23 μg/gm (n = 7) for OA synovium. Mast cell secretion was assessed and specific release of histamine from RA synovial mast cells was observed following stimulation with anti-IgE (32.3%), compound 48/80 (40.1%), calcium ionophore A23187 (25.2%), and a partially purified lymphokine with histamine-releasing activity (23.9%).

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