Detection of tumor necrosis factor α but not tumor necrosis factor β in rheumatoid arthritis synovial fluid and serum

Authors

  • T. Saxne MD, PhD,

    Corresponding author
    1. Departments of Rheumatology and Medical and Physiological Chemistry, University of Lund, Lund, Sweden; the Department of Molecular Biology and Immunology, Genentech, Inc., South San Francisco, California; the Department of Medicine, Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio; and the Audie L. Murphy Veterans Administration Hospital, San Antonio, Texas.
    • Department of Rheumatology, University Hospital, Lund S-221 85, Sweden
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  • M. A. Palladino Jr PhD,

    1. Departments of Rheumatology and Medical and Physiological Chemistry, University of Lund, Lund, Sweden; the Department of Molecular Biology and Immunology, Genentech, Inc., South San Francisco, California; the Department of Medicine, Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio; and the Audie L. Murphy Veterans Administration Hospital, San Antonio, Texas.
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  • D. Heinegãrd MD, PhD,

    Professor
    1. Departments of Rheumatology and Medical and Physiological Chemistry, University of Lund, Lund, Sweden; the Department of Molecular Biology and Immunology, Genentech, Inc., South San Francisco, California; the Department of Medicine, Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio; and the Audie L. Murphy Veterans Administration Hospital, San Antonio, Texas.
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  • N. Talal MD, PhD,

    Professor
    1. Departments of Rheumatology and Medical and Physiological Chemistry, University of Lund, Lund, Sweden; the Department of Molecular Biology and Immunology, Genentech, Inc., South San Francisco, California; the Department of Medicine, Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio; and the Audie L. Murphy Veterans Administration Hospital, San Antonio, Texas.
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  • F. A. Wollheim MD, PhD

    Professor
    1. Departments of Rheumatology and Medical and Physiological Chemistry, University of Lund, Lund, Sweden; the Department of Molecular Biology and Immunology, Genentech, Inc., South San Francisco, California; the Department of Medicine, Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio; and the Audie L. Murphy Veterans Administration Hospital, San Antonio, Texas.
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Abstract

Synovial fluids from 6 of 12 patients with rheumatoid arthritis (RA) and from 3 of 11 patients with reactive arthritis contained measurable levels of tumor necrosis factor α (TNFα). Seven of 12 sera from RA patients contained TNFα, while only 1 of those from reactive arthritis patients was positive. Gamma-inter-feron was detected in the synovial fluids and sera of only the RA patients. Tumor necrosis factor β was not detected in any sera or synovial fluids. RA patients with detectable TNFα had higher erythrocyte sedimentation rates and synovial fluid leukocyte counts.

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