Generation and Characterization of two Monoclonal Self-Associating IgG Rheumatoid Factors from a Rheumatoid Synovium
Article first published online: 9 DEC 2005
Copyright © 1992 American College of Rheumatology
Arthritis & Rheumatism
Volume 35, Issue 1, pages 101–105, January 1992
How to Cite
Lu, E. W., Deftos, M., Tighe, H., Carson, D. A. and Chen, P. P. (1992), Generation and Characterization of two Monoclonal Self-Associating IgG Rheumatoid Factors from a Rheumatoid Synovium. Arthritis & Rheumatism, 35: 101–105. doi: 10.1002/art.1780350115
- Issue published online: 9 DEC 2005
- Article first published online: 9 DEC 2005
- Manuscript Accepted: 20 AUG 1991
- Manuscript Received: 6 APR 1991
- NIH. Grant Numbers: AI-32243, AR-25443
Objective. To study IgG rheumatoid factor (RF) from rheumatoid synovium.
Methods. We fused the K6H6/B5 human—mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG-RF—secreting hybridomas.
Results. The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self-associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG-RFs in rheumatoid synovium.
Conclusion. IgG-RF—secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG-RF.