Type x collagen synthesis in human osteoarthritic cartilage. indication of chondrocyte hypertrophy
Article first published online: 9 DEC 2005
Copyright © 1992 American College of Rheumatology
Arthritis & Rheumatism
Volume 35, Issue 7, pages 806–811, July 1992
How to Cite
Von Der Mark, K., Kirsch, T., Nerlich, A., Kuss, A., Weseloh, G., Glückert, K. and Stöss, H. (1992), Type x collagen synthesis in human osteoarthritic cartilage. indication of chondrocyte hypertrophy. Arthritis & Rheumatism, 35: 806–811. doi: 10.1002/art.1780350715
- Issue published online: 9 DEC 2005
- Article first published online: 9 DEC 2005
- Manuscript Accepted: 20 FEB 1992
- Manuscript Received: 29 AUG 1991
- Bundesministerium für Forschung. Grant Number: (BMFT VM 08619/2)
Objective. To investigate the appearance of hypertrophic chondrocytes in osteoarthritic (OA) cartilage, using type X collagen as a specific marker.
Methods. The biosynthesis of type X collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with 3H-proline, immunoprecipitation, and sodium dodecyl sulfate–polyacrylamide gel electrophoresis of the synthesized collagens. Extracellular deposition of types X and II collagen was analyzed immunohistochemically.
Results. Immunostaining revealed an irregular distribution of type X collagen, which was localized around chondrocyte clusters in fibrillated OA cartilage, but was absent from the noncalcified region of normal articular cartilage. Freshly isolated OA chondrocytes synthesized predominantly type X collagen, while control chondrocytes synthesized mostly type II collagen.
Conclusion. Our findings indicate focal premature chondrocyte differentiation to hypertrophic cells in OA cartilage.