Lack of detection of enteroviral rna or bacterial dna in magnetic resonance imaging–directed muscle biopsies from twenty children with active untreated juvenile dermatomyositis
Article first published online: 9 DEC 2005
Copyright © 1995 American College of Rheumatology
Arthritis & Rheumatism
Volume 38, Issue 10, pages 1513–1518, October 1995
How to Cite
Pachman, L. M., Litt, D. L., Rowley, A. H., Hayford, J. R., Caliendo, J., Heller, S., Ticho, B. S., Christensen, M., Patterson, B., Ytterberg, S. R. and Pallansch, M. (1995), Lack of detection of enteroviral rna or bacterial dna in magnetic resonance imaging–directed muscle biopsies from twenty children with active untreated juvenile dermatomyositis. Arthritis & Rheumatism, 38: 1513–1518. doi: 10.1002/art.1780381019
- Issue published online: 9 DEC 2005
- Article first published online: 9 DEC 2005
- Manuscript Revised: 22 MAY 1995
- Manuscript Accepted: 22 MAY 1995
- Manuscript Received: 10 FEB 1995
- Marlene Apfelbaum Foundation
- NIAMS. Grant Number: P60-AR-30692
Objective. To investigate for the presence of increased titers of circulating antibody to putative infectious agents and for detectable viral RNA or bacterial DNA in children with active recent-onset juvenile der- matomyositis (DM).
Methods. Magnetic resonance imaging–directed muscle biopsies were performed in 20 children with active, untreated, recent-onset juvenile DM and in age-matched children with neurologic disease. Sera were tested for complement-fixing antibody to Coxsack-ievirus B (CVB), influenza A and B, parainfluenza 1 and 3, Mycoplasma pneumoniae, mumps, respiratory syncytial virus, and Reovirus; and by immunofluorescence for IgG antibody to Toxoplasma gondii cytomegalovirus and IgM antibody to Epstein-Barr virus. Muscle from juvenile DM patients and control children, CD-1 Swiss mice with and without CVB1 infection, and viral stock positive for CVB1–6 were tested using reverse-transcriptase polymerase chain reaction with 5 primer sets, 4 probes (1 Coxsackievirus, 3 Enterovirus), and universal primers for DNA.
Results. No increased antibody, viral RNA, or bacterial DNA was present in the juvenile DM patients or the control children.
Conclusion. Juvenile DM may be triggered by unidentified agent(s) in the genetically susceptible host.