The tight skin 2 mouse

Authors

  • Paul J. Christner PhD,

    1. Paul J. Christner, PhD, David Hawkins, BS, Linda D. Siracusa, PhD, Sergio A. Jiménez, MD: Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania
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  • Josephine Peters PhD,

    1. The MRC Radiobiology Unit, Chilton, Oxon, England
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  • David Hawkins BS,

    1. Paul J. Christner, PhD, David Hawkins, BS, Linda D. Siracusa, PhD, Sergio A. Jiménez, MD: Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania
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  • Linda D. Siracusa PhD,

    1. Paul J. Christner, PhD, David Hawkins, BS, Linda D. Siracusa, PhD, Sergio A. Jiménez, MD: Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania
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  • Sergio A. Jiménez MD

    Corresponding author
    1. Paul J. Christner, PhD, David Hawkins, BS, Linda D. Siracusa, PhD, Sergio A. Jiménez, MD: Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania
    • Thomas Jefferson University, Bluemle Life Sciences Building, 233 South 10th Street, Room 509, Philadelphia, PA 19107–5541
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Abstract

Objective. To describe the histopathologic and biochemical characteristics of skin from the Tsk2/ + mouse, a mutation with phenotypic features resembling those of systemic sclerosis (SSc), and to report the initial genetic mapping of the Tsk2 locus.

Methods. Histologic examination was performed and collagen content and type I collagen messenger RNA (mRNA) levels were determined in skin biopsy specimens from Tsk2/+ mice and normal mice. An intersubspecific backcross was conducted as a first step toward identifying the position of the Tsk2 locus on mouse chromosome 1.

Results. Histologic examination of Tsk2/+ mouse skin revealed marked accumulation of collagen and infiltration with mononuclear cells in the dermis and adipose tissue. Biochemical studies of Tsk2/+ mouse skin showed increased collagen content and elevated steady-state levels of α1(I) procollagen mRNA. Tsk2 was mapped to a 15.3-c entimorgan interval on mouse chromosome 1.

Conclusion. Tsk2 is a novel mutation which displays histopathologic and biochemical abnormalities similar to those present in the skin of patients with SSc, including increased collagen content and expression of type I collagen genes. This mutation has been mapped to a 15.3-c M region on mouse chromosome 1. Further study of this novel mutation will allow the identification of previously undescribed mechanisms involved in the regulation of normal and pathologic collagen gene expression.

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