Association of a functional CD19 polymorphism with susceptibility to systemic sclerosis
Article first published online: 8 DEC 2004
Copyright © 2004 by the American College of Rheumatology
Arthritis & Rheumatism
Volume 50, Issue 12, pages 4002–4007, December 2004
How to Cite
Tsuchiya, N., Kuroki, K., Fujimoto, M., Murakami, Y., Tedder, T. F., Tokunaga, K., Takehara, K. and Sato, S. (2004), Association of a functional CD19 polymorphism with susceptibility to systemic sclerosis. Arthritis & Rheumatism, 50: 4002–4007. doi: 10.1002/art.20674
- Issue published online: 8 DEC 2004
- Article first published online: 8 DEC 2004
- Manuscript Accepted: 26 AUG 2004
- Manuscript Received: 28 JUN 2004
- Health and Labor Sciences Research Grants from the Ministry of Health, Labor and Welfare of Japan
- Grant-in-Aid for Scientific Research on Priority Areas (C) “Medical Genome Science” from the Ministry of Education, Science, Sports and Culture of Japan
- Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science
CD19 is overexpressed in B cells from patients with systemic sclerosis (SSc), and plays a crucial role not only for autoantibody production, but also for skin fibrosis in mouse models. We previously reported the association of a GT repeat polymorphism in the 3′-untranslated region (3′-UTR) of CD19 with human systemic lupus erythematosus. In this study, we examined whether CD19 polymorphisms are associated with genetic susceptibility to SSc.
A case–control association study was performed for CD19 polymorphisms, −499G>T in the promoter region and a GT repeat polymorphism in the 3′-UTR, in 134 patients with SSc and 96 healthy individuals recruited at Kanazawa University. CD19 expression levels in the peripheral blood naive and memory B cells from SSc patients were examined by 2-color flow cytometry.
Carrier frequencies of the −499T allele in the promoter (odds ratio [OR] 2.18, 95% confidence interval [95% CI] 1.31–3.86, P = 0.003) and of the (GT)14 allele in the 3′-UTR (OR 1.86, 95% CI 1.05–3.28, P = 0.03) were significantly increased in SSc patients compared with healthy controls. Association was particularly evident in patients with limited cutaneous SSc with anticentromere antibodies. These alleles were in linkage disequilibrium, but the −499T allele seemed to play a primary role. CD19 expression levels in peripheral blood B cells were significantly elevated in both naive (P = 0.0029) and memory (P = 0.0022) B cells from patients with SSc who had the −499T allele as compared with those without the −499T allele.
The CD19 −499G>T polymorphism is associated with higher CD19 expression in B cells, and with susceptibility to SSc.