Dr. Wahren-Herlenius is coapplicant on a patent for an anti–HMGB-1 therapy for inflammation.
Increased extracellular levels of the novel proinflammatory cytokine high mobility group box chromosomal protein 1 in minor salivary glands of patients with Sjögren's syndrome
Article first published online: 26 JUN 2006
Copyright © 2006 by the American College of Rheumatology
Arthritis & Rheumatism
Volume 54, Issue 7, pages 2289–2294, July 2006
How to Cite
Ek, M., Popovic, K., Erlandsson Harris, H., Söderberg Nauclér, C. and Wahren-Herlenius, M. (2006), Increased extracellular levels of the novel proinflammatory cytokine high mobility group box chromosomal protein 1 in minor salivary glands of patients with Sjögren's syndrome. Arthritis & Rheumatism, 54: 2289–2294. doi: 10.1002/art.21969
- Issue published online: 27 JUN 2006
- Article first published online: 26 JUN 2006
- Manuscript Accepted: 12 APR 2006
- Manuscript Received: 11 JAN 2006
- Swedish Research Council
- Foundation for Strategic Research
- Karolinska Institutet
- Prof. Nanna Svartz' Foundation
- 80-Year Foundation of King Gustaf V
- Swedish Rheumatism Association
To investigate the expression of the novel proinflammatory cytokine high mobility group box chromosomal protein 1 (HMGB-1) in the salivary glands of patients with Sjögren's syndrome (SS) and patients with sicca symptoms.
Biopsy samples from the minor labial salivary glands of patients with SS, patients with sicca symptoms but no diagnosis of SS, and healthy controls were investigated. Expression of HMGB-1, tumor necrosis factor (TNF), and interleukin-1β (IL-1β) was analyzed using immunohistochemical staining on consecutive cryosections.
Increased expression of HMGB-1 was observed among the large infiltrates of mononuclear cells found in biopsy samples from patients with SS, and the degree of extracellular HMGB-1 was significantly higher in patients with SS compared with patients with sicca symptoms and with healthy controls (P < 0.05 and P < 0.01, respectively). Cellular expression of TNF was increased in patients with SS and in patients with sicca symptoms. In addition, the level of secreted TNF was significantly higher in patients with SS than in healthy controls (P < 0.05). Intracellular expression of IL-1β was detected in all groups, while extracellular IL-1β was observed almost exclusively among the infiltrating mononuclear cells of patients with SS.
The increased amount of extracellular HMGB-1 observed in salivary glands of patients with SS indicates that HMGB-1 is involved in the inflammatory process of the disease. This cytokine, along with TNF and IL-1β, may form a proinflammatory loop that promotes the chronic features of the glandular inflammation in SS.