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Research Article
Role of aggrecanase 1 in Lyme arthritis
Article first published online: 28 SEP 2006
DOI: 10.1002/art.22128
Copyright © 2006 by the American College of Rheumatology
1529-0131/asset/cover.gif?v=1&s=104d5c2bb8ef72deba26790b855af7ab80697a0e "Arthritis & Rheumatism")
Additional Information
How to Cite
Behera, A. K., Hildebrand, E., Szafranski, J., Hung, H.-H., Grodzinsky, A. J., Lafyatis, R., Koch, A. E., Kalish, R., Perides, G., Steere, A. C. and Hu, L. T. (2006), Role of aggrecanase 1 in Lyme arthritis. Arthritis & Rheumatism, 54: 3319–3329. doi: 10.1002/art.22128
Publication History
- Issue published online: 28 SEP 2006
- Article first published online: 28 SEP 2006
- Manuscript Accepted: 26 JUN 2006
- Manuscript Received: 16 FEB 2006
Funded by
- American Lung Association
- Earle P. Charlton Research Fund
- Natalie V. Zucker Research Center for Women Scholars
- NIH. Grant Numbers: AR-45779, R01-AI-40987, R01-AR-48267, AR-20358, R01-AI-44240, R01-AI-50043, U01-AI-058266
- Department of Veterans Affairs
- Frederick G. L. Huetwell and William D. Robinson, MD, Professorship in Rheumatology
- Abstract
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- References
- Cited By
Abstract
Objective
Arthritis is one of the hallmarks of late-stage Lyme disease. Previous studies have shown that infection with Borrelia burgdorferi, the causative agent of Lyme disease, results in degradation of proteoglycans and collagen in cartilage. B burgdorferi do not appear to produce any exported proteases capable of digesting proteoglycans and collagen, but instead, induce and activate host proteases, such as matrix metalloproteinases (MMPs), which results in cartilage degradation. The role of aggrecanases in Lyme arthritis has not yet been determined. We therefore sought to delineate the contribution of aggrecanases to joint destruction in Lyme arthritis.
Methods
We examined the expression patterns of aggrecanases 1 and 2 (ADAMTS 4 and 5, respectively) in B burgdorferi–infected primary human chondrocyte cell cultures, in synovial fluid samples from patients with active Lyme arthritis, and in the joints of mice by real-time quantitative reverse transcription–polymerase chain reaction and immunoblotting techniques. Bovine cartilage explants were used to determine the role of aggrecanases in B burgdorferi–induced cartilage degradation.
Results
ADAMTS-4, but not ADAMTS-5, was induced in human chondrocytes infected with B burgdorferi. The active forms of ADAMTS-4 were increased in synovial fluid samples from patients with active Lyme arthritis and were elevated in the joints of mice infected with B burgdorferi. Using cartilage explant models of Lyme arthritis, it appeared that the cleavage of aggrecan was predominantly mediated by “aggrecanases” rather than MMPs.
Conclusion
The induction of ADAMTS-4 by B burgdorferi results in the cleavage of aggrecan, which may be an important first step that leads to permanent degradation of cartilage.