p53 Controls Autoimmune Arthritis via STAT-Mediated Regulation of the Th17 Cell/Treg Cell Balance in Mice
Article first published online: 28 MAR 2013
Copyright © 2013 by the American College of Rheumatology
Arthritis & Rheumatism
Volume 65, Issue 4, pages 949–959, April 2013
How to Cite
Park, J.-S., Lim, M.-A., Cho, M.-L., Ryu, J.-G., Moon, Y.-M., Jhun, J.-Y., Byun, J.-K., Kim, E.-K., Hwang, S.-Y., Ju, J. H., Kwok, S.-K. and Kim, H.-Y. (2013), p53 Controls Autoimmune Arthritis via STAT-Mediated Regulation of the Th17 Cell/Treg Cell Balance in Mice. Arthritis & Rheumatism, 65: 949–959. doi: 10.1002/art.37841
- Issue published online: 28 MAR 2013
- Article first published online: 28 MAR 2013
- Accepted manuscript online: 28 DEC 2012 11:29AM EST
- Manuscript Accepted: 18 DEC 2012
- Manuscript Received: 23 AUG 2012
- Public Welfare and Safety Research Program through the National Research Foundation of Korea
- Ministry of Education, Science, and Technology. Grant Number: 2010-0020767
- National Project for Personalized Genomic Medicine
- Ministry for Health and Welfare, Republic of Korea. Grant Number: A111218-PG01
To investigate the connection between p53 and interleukin-17–producing Th17 cell/Treg cell balance in rheumatoid arthritis (RA).
Th17 cell and Treg cell frequencies were analyzed by flow cytometry, and cytokine levels in the supernatant were determined using enzyme-linked immunosorbent assays. The expression of transcription factors was analyzed by immunostaining and Western blotting, and the interactions between p53 and STAT-3 or STAT-5 were determined by immunoprecipitation–Western blot analysis. A p53 agonist was administered in the collagen-induced arthritis (CIA) model, and the effects in vivo were determined.
CD4+ T cells from p53–/– mice decreased the activity of STAT-5, lowered the level of phosphorylated STAT-5, and compromised Treg cell differentiation. The protein p53 bound STAT-5 directly, and this interaction was enhanced with increasing p53 activity. Under inflammatory conditions, p53 suppressed Th17 cell differentiation and skewed T cells toward Treg cell differentiation through the activation of STAT-5 signaling cascades. In mice with CIA, injection of a p53 overexpression vector or an antagonist of Mdm2 had the effect of controlling arthritis development in vivo. The regulatory effect of p53 was recapitulated in the cells of RA patients, with more pronounced suppression due to the repressed status of p53 in RA.
We demonstrated a link between p53-mediated and STAT-mediated regulation of Th17 cells/Treg cells in RA. Our results suggest that factors involved in this pathway might constitute novel therapeutic targets for the treatment of RA.