Dr. Licha is the inventor of this technique.
Optoacoustic Imaging and Staging of Inflammation in a Murine Model of Arthritis†
Article first published online: 28 JUL 2014
Copyright © 2014 by the American College of Rheumatology
Arthritis & Rheumatology
Volume 66, Issue 8, pages 2071–2078, August 2014
How to Cite
Beziere, N., von Schacky, C., Kosanke, Y., Kimm, M., Nunes, A., Licha, K., Aichler, M., Walch, A., Rummeny, E. J., Ntziachristos, V. and Meier, R. (2014), Optoacoustic Imaging and Staging of Inflammation in a Murine Model of Arthritis. Arthritis & Rheumatology, 66: 2071–2078. doi: 10.1002/art.38642
Mivenion GmbH holds patent WO2011095311 for dPGS and its use as a targeting moiety for near-infrared imaging.
- Issue published online: 28 JUL 2014
- Article first published online: 28 JUL 2014
- Accepted manuscript online: 1 APR 2014 12:00AM EST
- Manuscript Accepted: 20 MAR 2014
- Manuscript Received: 27 SEP 2013
- European Union Seventh Framework Programme
- Research and Technology Development. Grant Number: 2007-2013 FMT-XCT
- Cluster of Excellence project Nanosystem Initiative Munich
- BMBF (Cluster of Excellence project M4 Individualized Medicine)
- SFB. Grant Number: 824
- Project Z2
Rheumatoid arthritis (RA) is one of the most frequent inflammatory diseases, causing pain and disability in the affected joints. Early diagnosis is essential for the efficiency of symptom-targeting treatments, but its diagnosis requires careful clinical, serologic, and imaging examinations, such as magnetic resonance imaging (MRI), which is both expensive and time consuming. In an effort to provide the biomedical community with a more accessible way to assess the advancement of arthritis, this study sought to investigate the use of multispectral optoacoustic tomography (MSOT) in a murine arthritis model, to visualize the extent of inflammation in vivo through an L-selectin/P-selectin–targeting contrast agent.
Mice with collagen-induced arthritis were studied as a model of RA. MSOT was performed using an L-selectin/P-selectin–targeting contrast agent, polyanionic dendritic polyglycerol sulfate (dPGS) labeled with a near-infrared (NIR) fluorophore, to increase the contrast of the arthritic joint. The signal intensity ratios between healthy legs and arthritic legs were calculated. Findings on contrast-enhanced MRI, clinical observations, the lymphocyte:granulocyte ratio, and histologic findings served as referents for comparison.
MSOT using an inflammation-targeting contrast agent, dPGS-NIR, allowed for accurate diagnosis of inflammation in the mouse joints. In addition, use of this technique resulted in significant differentiation of the inflamed joints from the healthy joints (P = 0.023). The observed advancement of arthritis on the MSOT images was confirmed by clinical observation, blood analysis, contrast-enhanced MRI, and ex vivo histologic examinations.
This study demonstrates that the combination of an inflammation-targeting contrast agent and optoacoustic tomographic imaging presents a promising means for the diagnosis of RA and the staging of arthritis-related inflammation.