[Ru(bpy)2(dcbpy)NHS] Labeling/Aptamer-Based Biosensor for the Detection of Lysozyme by Increasing Sensitivity with Gold Nanoparticle Amplification

Authors

  • Jianguo Bai,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (China), Fax: (+86) 431 8568 9711
    2. College of Chemistry, Jilin University, Changchun, 130022 (China), Fax: (+86) 431 8509 5144
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  • Hui Wei,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (China), Fax: (+86) 431 8568 9711
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  • Bingling Li,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (China), Fax: (+86) 431 8568 9711
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  • Lihua Song,

    1. College of Chemistry, Jilin University, Changchun, 130022 (China), Fax: (+86) 431 8509 5144
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  • Lanyun Fang,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (China), Fax: (+86) 431 8568 9711
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  • Zhaozi Lv,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (China), Fax: (+86) 431 8568 9711
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  • Weihong Zhou Prof. Dr.,

    1. College of Chemistry, Jilin University, Changchun, 130022 (China), Fax: (+86) 431 8509 5144
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  • Erkang Wang Prof. Dr.

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (China), Fax: (+86) 431 8568 9711
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Abstract

A novel [Ru(bpy)2(dcbpy)NHS] labeling/aptamer-based biosensor combined with gold nanoparticle amplification for the determination of lysozyme with an electrochemiluminescence (ECL) method is presented. In this work, an aptamer, an ECL probe, gold nanoparticle amplification, and competition assay are the main protocols employed in ECL detection. With all the protocols used, an original biosensor coupled with an aptamer and [Ru(bpy)2(dcbpy)NHS] has been prepared. Its high selectivity and sensitivity are the main advantages over other traditional [Ru(bpy)3]2+ biosensors. The electrochemical impedance spectroscopy (EIS) and atomic force microscopy (AFM) characterization illustrate that this biosensor is fabricated successfully. Finally, the biosensor was applied to a displacement assay in different concentrations of lysozyme solution, and an ultrasensitive ECL signal was obtained. The ECL intensity decreased proportionally to the lysozyme concentration over the range 1.0×10−13–1.0×10−8 mol L−1 with a detection limit of 1.0×10−13 mol L−1. This strategy for the aptasensor opens a rapid, selective, and sensitive route for the detection of lysozyme and potentially other proteins.

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