Zinc(II)-Dipicolylamine-Functionalized Polydiacetylene–Liposome Microarray: A Selective and Sensitive Sensing Platform for Pyrophosphate Ions

Authors

  • Kyung Mi Kim,

    1. Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), San 31 Hyoja-dong, Pohang 790-784 (Korea), Fax: (+82) 54-279-3399
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  • Dong Ju Oh,

    1. Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), San 31 Hyoja-dong, Pohang 790-784 (Korea), Fax: (+82) 54-279-3399
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  • Prof. Kyo Han Ahn

    Corresponding author
    1. Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), San 31 Hyoja-dong, Pohang 790-784 (Korea), Fax: (+82) 54-279-3399
    • Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), San 31 Hyoja-dong, Pohang 790-784 (Korea), Fax: (+82) 54-279-3399

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Abstract

A microarray-chip assay system for the fluorescence detection of phosphate-containing analytes in aqueous media has been constructed from stimuli-responsive polymerized poly(diacetylene)-liposomes for the first time. Proper combination of the liposome components (ZnII-dipicolylamine for phosphate binding and an amine-terminated component for anchoring the liposome onto an aldehyde-derivatized glass plate), has led to a microarray chip that selectively detects pyrophosphate, an important biomarker, over competing anions, such as phosphate and adenosine triphosphate, with nanomolar sensitivity. The chip-based assay shows advantages, such as high specificity and sensitivity, over solution-based assays that use the same liposomes, and over known homogeneous molecular sensing systems.

Abstract

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