The tunable excited-state properties of a new donor–π-acceptor–π-donor-type fluorophore with a bipyridyl moiety and its ability to respond to different analytes in solution and on paper microchannels are described. Furthermore, the multiple analyte response of fluorophore has been exploited to perform multiple logic operations. Molecule , by virtue of its excited-state charge transfer, exhibits solvatochromism and reversible modulation of its emission in response to multiple chemical inputs, thus resulting in different fluorescent signals. The intraligand charge-transfer (ILCT) emission of at 574 nm has been modulated to three emission outputs by using different chemical inputs, such as Zn2+, H+, and ethylenediaminetetraacetic acid (EDTA). Thus, different logic operations such as AND, 2-input-INH, 3-input-INH, IMP, and a combination of these logic operations could be achieved.