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Enzyme-Responsive Multifunctional Magnetic Nanoparticles for Tumor Intracellular Drug Delivery and Imaging

Authors

  • Yanmei Yang,

    1. Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore), Fax: (+65) 6791-1961
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  • Junxin Aw,

    1. Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore), Fax: (+65) 6791-1961
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  • Kai Chen,

    1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Standford University Medical Center, California, 94305 (USA)
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  • Fang Liu,

    1. Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore), Fax: (+65) 6791-1961
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  • Dr. Parasuraman Padmanabhan,

    1. Translation Molecular Imaging Group (TMIG), Singapore, Bio-Imaging Consortium (SBIC), A*Star, Singapore 138667 (Singapore)
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  • Prof. Dr. Yanglong Hou,

    1. Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, Beijing 100871 (China)
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  • Prof. Dr. Zhen Cheng,

    Corresponding author
    1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Standford University Medical Center, California, 94305 (USA)
    • Zhen Cheng, Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Standford University Medical Center, California, 94305 (USA)

      Bengang Xing, Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore), Fax: (+65) 6791-1961

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  • Prof. Dr. Bengang Xing

    Corresponding author
    1. Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore), Fax: (+65) 6791-1961
    • Zhen Cheng, Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Standford University Medical Center, California, 94305 (USA)

      Bengang Xing, Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore), Fax: (+65) 6791-1961

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Abstract

Enzyme-responsive, hybrid, magnetic silica nanoparticles have been employed for multifunctional applications in selective drug delivery and intracellular tumor imaging. In this study, doxorubicin (Dox)-conjugated, enzyme-cleavable peptide precursors were covalently tethered onto the surface of uniform silica-coated magnetic nanoparticles through click chemistry. This enzyme-responsive nanoparticle conjugate demonstrated highly efficient Dox release upon specific enzyme interactions in vitro. It also exhibits multiple functions in selective tumor intracellular drug delivery and imaging in the tumor cells with high cathepsin B expression, whereas it exhibited lower cytotoxicity towards other cells without enzyme expression.

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