SEARCH

SEARCH BY CITATION

Keywords:

  • cell culture;
  • hydrogels;
  • self-assembly;
  • supramolecular chemistry;
  • zwitterions

Abstract

Supramolecular hydrogels constructed through molecular self-assembly of small molecules have unique stimuli-responsive properties; however, they are mechanically weak in general, relative to conventional polymer gels. Very recently, we developed a zwitterionic amino acid tethered amphiphilic molecule 1, which gave rise to a remarkably stiff hydrogel comparable with polymer-based agarose gel, retaining reversible thermal-responsive properties. In this study, we describe that rational accumulation of multiple and orthogonal noncovalent interactions in the supramolecular nanofibers of 1 played crucial roles not only in the mechanical reinforcement but also in the multistimuli responsiveness. That is, the zwitterionic amino acid moiety and the C[BOND]C double bond unit of the hydrogelator 1 can function as a pH-responsive unit and a light-responsive unit, respectively. We also demonstrated that this stiff and multistimuli-responsive supramolecular hydrogel 1 is applied as a unique mold for 2D and 3D-patterning of various substances. More significantly, we succeeded in the fabrication of a collagen gel for spatial patterning, culturing, and differentiation of live cells by using hydrogel 1 molds equipped with 2D/3D microspace channels (100–200 μm in diameter).

Featured Compounds

VIEW

  1. 1 - 7
Compound 1
Molecular Weight:509.5925
Molecular Formula:C25H39N3O8
InChIKey:CXZHZHIVIYRNDI-ICSRWYNCSA-N
View compound in article | Full details | Search for this compound
Compound 2
Molecular Weight:655.7337
Molecular Formula:C31H49N3O12
InChIKey:Not Available
View compound in article | Full details | Search for this compound
Compound 3
Molecular Weight:657.7495
Molecular Formula:C31H51N3O12
InChIKey:Not Available
View compound in article | Full details | Search for this compound
Compound 4
Molecular Weight:537.6456
Molecular Formula:C27H43N3O8
InChIKey:LWWXQKDBANYHFV-ZUMAOHNSSA-N
View compound in article | Full details | Search for this compound
Compound 5
Molecular Weight:537.6456
Molecular Formula:C27H43N3O8
InChIKey:QYICHBFDOFTYHA-WQHODERWSA-N
View compound in article | Full details | Search for this compound
Compound 6
Molecular Weight:537.6456
Molecular Formula:C27H43N3O8
InChIKey:LWWXQKDBANYHFV-FSUQMGBSSA-N
View compound in article | Full details | Search for this compound
Compound 7
Molecular Weight:551.6722
Molecular Formula:C28H45N3O8
InChIKey:JJCGRMBQDPHYHR-ZIDQQFFPSA-N
View compound in article | Full details | Search for this compound

VIEW

  1. 1 - 7