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An Anticoagulant Activity System Using Nanoengineered Autofluorescent Heparin Nanotubes

Authors

  • Wei Cui,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Bei Yi Jie, Zhong Guan Cun, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
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  • Dr. Yue Cui,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Bei Yi Jie, Zhong Guan Cun, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
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  • Dr. Pengli Zhu,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Bei Yi Jie, Zhong Guan Cun, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
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  • Dr. Jie Zhao,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Bei Yi Jie, Zhong Guan Cun, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
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  • Dr. Ying Su,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Bei Yi Jie, Zhong Guan Cun, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
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  • Dr. Yang Yang,

    1. National Center for Nanoscicence and Technology, No. 11, Bei Yi Tiao, Zhong Guan Cun, Beijing, 100190 (P.R. China)
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  • Prof. Dr. Junbai Li

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Bei Yi Jie, Zhong Guan Cun, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
    • Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Bei Yi Jie, Zhong Guan Cun, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629

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Abstract

Heparin (HEP) and periodate-oxidized heparin (O-HEP) nanotubes were prepared by combining the template method with a layer-by-layer (LbL) technique. The tubular structure was obtained and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and confocal laser scanning microscopy (CLSM). O-HEP is one of the HEP derivatives that contains anticoagulant activity and preserves its ability for other effects. Chitosan (CHI) and O-HEP have been used to fabricate nanotubes by covalent cross-linking Schiff base reactions. It is demonstrated that the obtained nanotubes have the significant feature of autofluorescence without the addition of any fluorescent dyes and they retain their anticoagulation activity. Compared with O-HEP/CHI nanotubes, HEP/CHI nanotubes show high anticoagulation activity and do not have autofluorescence. Furthermore, this method could be extended to other copolysaccharide derivatives for the preparation of autofluorescent nanomaterials.

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