Single-Step Turn-On Homogeneous Fluorescent Immunosensor for Rapid, Sensitive, and Selective Detection of Proteins

Authors

  • Dan Wang,

    1. Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China), Fax: (+86) 10-62751708
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  • Xiaocui Zhu,

    1. Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China), Fax: (+86) 10-62751708
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  • Dongqin Zhao,

    1. Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China), Fax: (+86) 10-62751708
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  • Yingxiao Cai,

    1. Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China), Fax: (+86) 10-62751708
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  • Prof. Dr. Meiping Zhao

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China), Fax: (+86) 10-62751708
    • Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China), Fax: (+86) 10-62751708

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Abstract

original image

Quick & easy: A simple and positive-readout fluorescent immunosensor was developed based on a quencher composed of a 3 nm gold nanoparticle (GNP) covalently linked to a Fab fragment, which proved to be stable and specific. The method allows for direct and sensitive detection of target antigens in homogeneous solutions in one step without any separation steps. It has been successfully applied to rapidly quantify the amount of secretory immunoglobulin A (sIgA) in human saliva samples.

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