Nanowire Transistor-Based Ultrasensitive Virus Detection with Reversible Surface Functionalization
Article first published online: 19 JUN 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Chemistry – An Asian Journal
Volume 7, Issue 9, pages 2073–2079, September 2012
How to Cite
Chiang, P.-L., Chou, T.-C., Wu, T.-H., Li, C.-C., Liao, C.-D., Lin, J.-Y., Tsai, M.-H., Tsai, C.-C., Sun, C.-J., Wang, C.-H., Fang, J.-M. and Chen, Y.-T. (2012), Nanowire Transistor-Based Ultrasensitive Virus Detection with Reversible Surface Functionalization. Chem. Asian J., 7: 2073–2079. doi: 10.1002/asia.201200222
- Issue published online: 22 AUG 2012
- Article first published online: 19 JUN 2012
- Manuscript Revised: 16 APR 2012
- Manuscript Received: 9 MAR 2012
- National Science Council of Taiwan. Grant Numbers: NSC97-2627M-002-019, NSC97-2628M-002-013-MY3
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- reversible surface functionalization;
We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAbH5/SiNW-FET) after detecting approximately 10−17 M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles.