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Nanowire Transistor-Based Ultrasensitive Virus Detection with Reversible Surface Functionalization

Authors

  • Pei-Ling Chiang,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Tzu-Chi Chou,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
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  • Tzu-Heng Wu,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Chang-Chi Li,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Dr. Chun-Da Liao,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Dr. Jeng-Yu Lin,

    1. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Ming-Hsueh Tsai,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Dr. Chia-Chang Tsai,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Chih-Jung Sun,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
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  • Prof. Dr. Ching-Ho Wang,

    1. Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C)
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  • Prof. Dr. Jim-Min Fang,

    Corresponding author
    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. The Genomics Research Center, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
    • Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812

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  • Prof. Dr. Yit-Tsong Chen

    Corresponding author
    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812
    2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C)
    • Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812

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Abstract

We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAbH5/SiNW-FET) after detecting approximately 10−17M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles.

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