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Keywords:

  • enzyme models;
  • heme proteins;
  • human serum albumin;
  • peroxidase;
  • recombinant proteins
Thumbnail image of graphical abstract

Cooking up enzymes: Iron protoporphyrin IX (heme) is bound within a hydrophobic cavity in subdomain IB of human serum albumin (HSA). Site-specific mutations to introduce proximal and distal histidines into the heme pocket of HSA conferred a peroxidase activity to the prosthetic heme group. The catalytic activity of the recombinant HSA(mutant)–heme complex for guaiacol oxidation is 17-fold higher than that of HSA(wild type)–heme.