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Real-Time Fluorescence Turn-On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe

  1. Jian Chen1,2,
  2. Huping Jiao1,2,
  3. Wenying Li1,2,
  4. Dongli Liao1,2,
  5. Dr. Huipeng Zhou1,2,
  6. Prof. Dr. Cong Yu1,2,*

Article first published online: 9 NOV 2012

DOI: 10.1002/asia.201200880

Issue

Chemistry – An Asian Journal

Chemistry – An Asian Journal

Volume 8, Issue 1, pages 276–281, January 2013

Additional Information

How to Cite

Chen, J., Jiao, H., Li, W., Liao, D., Zhou, H. and Yu, C. (2013), Real-Time Fluorescence Turn-On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe. Chem. Asian J., 8: 276–281. doi: 10.1002/asia.201200880

Author Information

  1. 1

    State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)

  2. 2

    Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P. R. China), Fax: (+86) 431-8526-2710

*State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)

Publication History

  1. Issue published online: 20 DEC 2012
  2. Article first published online: 9 NOV 2012
  3. Manuscript Received: 21 SEP 2012

Funded by

  • Chinese Academy of Sciences
  • National Natural Science Foundation of China. Grant Numbers: 21075119, 91027036, 21275139
  • National Basic Research Program of China. Grant Number: 2011CB911002
  • Changchun Municipal Bureau of Science and Technology. Grant Number: 2011225
  • State Key Laboratory of Supramolecular Structure and Materials. Grant Number: SKLSSM201215

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Keywords:

  • aggregation;
  • alkaline phosphatase;
  • biosensors;
  • fluorescence;
  • perylene probe

Abstract

A tetracationic perylene probe (probe 2) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.

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Featured Compounds

Compound 1
Molecular Weight:885.1818
Molecular Formula:C52H76N4O8
InChIKey:LRMIQIIAPYHYFR-UHFFFAOYSA-N
View compound in article | Full details | Search this compound
Compound 2
Molecular Weight:968.357
Molecular Formula:C40H40Br4O8
InChIKey:YNEPQZUFEVVCNN-UHFFFAOYSA-N
View compound in article | Full details | Search this compound
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