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Real-Time Fluorescence Turn-On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe

Authors

  • Jian Chen,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P. R. China), Fax: (+86) 431-8526-2710
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  • Huping Jiao,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P. R. China), Fax: (+86) 431-8526-2710
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  • Wenying Li,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P. R. China), Fax: (+86) 431-8526-2710
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  • Dongli Liao,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P. R. China), Fax: (+86) 431-8526-2710
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  • Dr. Huipeng Zhou,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P. R. China), Fax: (+86) 431-8526-2710
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  • Prof. Dr. Cong Yu

    Corresponding author
    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P. R. China), Fax: (+86) 431-8526-2710
    • State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P. R. China)

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Abstract

A tetracationic perylene probe (probe 2) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.

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