Increased Efficacies in Magnetofection and Gene Delivery to Hepatocellular Carcinoma Cells with Ternary Organic–Inorganic Hybrid Nanocomposites

Authors

  • Prof. Dr. Ken Cham-Fai Leung,

    Corresponding author
    1. Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (P. R. China), Fax: (+852) 3411-7348
    2. Institute of Creativity, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (P. R. China)
    3. Institute of Molecular Functional Materials, Areas of Excellence, University Grants Committee, Hong Kong SAR (P. R. China)
    • Ken Cham-Fai Leung, Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (P. R. China), Fax: (+852) 3411-7348

      Christopher H. K. Cheng, School of Biomedical Sciences and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)

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  • Dr. Chun-Pong Chak,

    1. Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)
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  • Siu-Fung Lee,

    1. Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)
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  • Josie M. Y. Lai,

    1. School of Biomedical Sciences and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)
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  • Dr. Xiao-Ming Zhu,

    1. Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (P. R. China), Fax: (+852) 3411-7348
    2. Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)
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  • Prof. Dr. Yi-Xiang J. Wang,

    1. Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)
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  • Kathy W. Y. Sham,

    1. School of Biomedical Sciences and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)
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  • Chi-Hin Wong,

    1. Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (P. R. China), Fax: (+852) 3411-7348
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  • Prof. Dr. Christopher H. K. Cheng

    Corresponding author
    1. School of Biomedical Sciences and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)
    • Ken Cham-Fai Leung, Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (P. R. China), Fax: (+852) 3411-7348

      Christopher H. K. Cheng, School of Biomedical Sciences and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (P. R. China)

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Abstract

original image

A magnetic personality: Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic–inorganic hybrid nanocomposites that contain deferoxamine-coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellular uptake efficacies depend on the ratio of components in PEI/DNA/NP, as assessed by Prussian staining, magnetic resonance imaging, and luciferase expression in the presence and absence of a magnet during transfection.

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