Key Structural Elements of Unsymmetrical Cyanine Dyes for Highly Sensitive Fluorescence Turn-On DNA Probes (pages 233–238)
Kakishi Uno, Taeko Sasaki, Nagisa Sugimoto, Dr. Hideto Ito, Dr. Taishi Nishihara, Dr. Shinya Hagihara, Prof. Dr. Tetsuya Higashiyama, Dr. Narie Sasaki, Dr. Yoshikatsu Sato and Prof. Dr. Kenichiro Itami
Version of Record online: 16 DEC 2016 | DOI: 10.1002/asia.201601430
Amino magic: A systematic structure–property relationship study has revealed that the dialkylamino group at the 2-position of quinoline in unsymmetrical cyanine dyes (see figure) plays a critical role in enhancement of the fluorescence upon binding to double-stranded DNA. The newly developed dyes were also able to stain the nucleus specifically in fixed HeLa cells examined by using a confocal laser-scanning microscope.