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Molecular Characterisation of Gastrointestinal Microbiota of Children With Autism (With and Without Gastrointestinal Dysfunction) and Their Neurotypical Siblings

Authors

  • Shakuntla V. Gondalia,

    Corresponding author
    • Swinburne Autism Bio-Research Initiative (SABRI), Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
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  • Enzo A. Palombo,

    1. Swinburne Autism Bio-Research Initiative (SABRI), Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
    2. Environment and Biotechnology Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
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  • Simon R. Knowles,

    1. Swinburne Autism Bio-Research Initiative (SABRI), Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
    2. Brain and Psycological Sciences Research Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
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  • Stephen B. Cox,

    1. Research and Testing Labs, Lubbock, Texas
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  • Denny Meyer,

    1. Psychological Sciences and Statistics, Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
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  • David W. Austin

    1. Swinburne Autism Bio-Research Initiative (SABRI), Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
    2. Brain and Psycological Sciences Research Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia
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Address for correspondence and reprints: Shakuntla V. Gondalia, Swinburne Autism Bio-Research Initiative (SABRI), Faculty of Life and Social Sciences, Swinburne University of Technology, Mail H31, Hawthorn, Vic. 3122, Australia. E-mail: sgondalia@swin.edu.au

Abstract

Many children with autism spectrum disorders (ASDs) suffer from gastrointestinal problems such as diarrhoea, constipation and abdominal pain. This has stimulated investigations into possible abnormalities of intestinal microbiota in autistic patients. Therefore, we designed this study to identify differences (and/or similarities) in the microbiota of children with autism (without gastrointestinal dysfunction: n = 23; with gastrointestinal dysfunction: n = 28) and their neurotypical siblings (n = 53) who share a similar environment using bacterial tag-encoded FLX amplicon pyrosequencing. Regardless of the diagnosis and sociodemographic characteristics, overall, Firmicutes (70%), Bacteroidetes (20%) and Proteobacteria (4%) were the most dominant phyla in samples. Results did not indicate clinically meaningful differences between groups. The data do not support the hypothesis that the gastrointestinal microbiota of children with ASD plays a role in the symptomatology of ASD. Other explanations for the gastrointestinal dysfunction in this population should be considered including elevated anxiety and self-restricted diets. Autism Res 2012, 5: 419–427. © 2012 International Society for Autism Research, Wiley Periodicals, Inc.

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