Feasibility and relevance of examining lymphoblastoid cell lines to study role of microRNAs in autism
Article first published online: 15 SEP 2008
Copyright © 2008, International Society for Autism Research, Wiley-Liss, Inc.
Volume 1, Issue 4, pages 240–250, August 2008
How to Cite
Talebizadeh, Z., Butler, M. G. and Theodoro, M. F. (2008), Feasibility and relevance of examining lymphoblastoid cell lines to study role of microRNAs in autism. Autism Res, 1: 240–250. doi: 10.1002/aur.33
- Issue published online: 9 OCT 2008
- Article first published online: 15 SEP 2008
- Manuscript Accepted: 9 AUG 2008
- Manuscript Received: 14 MAY 2008
- Children's Mercy Hospital (CMH) Physician Scientist Award. Grant Number: 01.3905
- Cure Autism Now (CAN) Foundation. Grant Number: 01.3956
- lymphoblastoid cell lines;
- differential expression
To assess the feasibility and relevance of using lymphoblastoid cell lines to study the role of noncoding RNAs in the etiology of autism, we evaluated global expression profiling of 470 mature human microRNAs from six subjects with autism compared with six matched controls. Differential expression (either higher or lower) for 9 of the 470 microRNAs was observed in our autism samples compared with controls. Potential target genes for these microRNAs were identified using computer tools, which included several autism susceptibility genes. Our preliminary results indicate microRNAs should be considered and evaluated in the etiology of autism. In addition, analysis of this class of noncoding RNAs in lymphoblastoid cells has the potential to reveal at least a subset of brain-related microRNAs implicated in autism. Subsequently, this model system should allow for detection of complex subtle changes in susceptibility genes/pathways contributing to autism.