Properties of chitosan-immobilized cellulase in ionic liquid


Address for correspondence: Professor Shitao Yu, Department of Biology, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China. Tel: +86-532-84022879; Fax: +86-532-84022719; e-mail:


The properties of cellulase that was attached to the surface of the chitosan carrier in aqueous–ionic liquid (IL; 1,3-dimeth-ylimidazolium dimethylphosphate) mixture were studied. The optimal temperature for immobilized cellulase in aqueous–IL mixed solutions was 60 °C. The immobilized cellulase acquired the highest relative activity at a ratio of 1:4 (IL to water, v/v), compared to activity levels of 79% and 7%, when the ratio of IL to water (v/v) was 0:1 and 1:0, respectively. At 80 °C, the immobilized cellulase in the aqueous–IL mixture conserved 46.3% activity after 120 Min. The immobilized cellulase can be effectively reused three times. After 4 weeks, the activity of immobilized cellulase maintained 83.5%. The Michaelis constant (Km) and maximum reaction velocity (Vm) values for the immobilized cellulase were 4.8 mg/L and 0.156 mg/(mL Min), respectively. To the best of our knowledge, this is the first report on the properties of chitosan-immobilized cellulase in aqueous–IL.