Properties of chitosan-immobilized cellulase in ionic liquid
Article first published online: 11 JAN 2013
© 2013 International Union of Biochemistry and Molecular Biology, Inc.
Biotechnology and Applied Biochemistry
Volume 60, Issue 2, pages 231–235, March/April 2013
How to Cite
Su, Z., Yu, Y., Liang, C., Li, L. and Yu, S. (2013), Properties of chitosan-immobilized cellulase in ionic liquid. Biotechnology and Applied Biochemistry, 60: 231–235. doi: 10.1002/bab.1057
- Issue published online: 18 APR 2013
- Article first published online: 11 JAN 2013
- Manuscript Accepted: 24 OCT 2012
- Manuscript Received: 29 AUG 2012
- National Natural Science Foundation of China. Grant Numbers: 30900126, 31070520
- ionic liquid;
The properties of cellulase that was attached to the surface of the chitosan carrier in aqueous–ionic liquid (IL; 1,3-dimeth-ylimidazolium dimethylphosphate) mixture were studied. The optimal temperature for immobilized cellulase in aqueous–IL mixed solutions was 60 °C. The immobilized cellulase acquired the highest relative activity at a ratio of 1:4 (IL to water, v/v), compared to activity levels of 79% and 7%, when the ratio of IL to water (v/v) was 0:1 and 1:0, respectively. At 80 °C, the immobilized cellulase in the aqueous–IL mixture conserved 46.3% activity after 120 Min. The immobilized cellulase can be effectively reused three times. After 4 weeks, the activity of immobilized cellulase maintained 83.5%. The Michaelis constant (Km) and maximum reaction velocity (Vm) values for the immobilized cellulase were 4.8 mg/L and 0.156 mg/(mL Min), respectively. To the best of our knowledge, this is the first report on the properties of chitosan-immobilized cellulase in aqueous–IL.