Prenatal choline supplementation mitigates behavioral alterations associated with prenatal alcohol exposure in rats
Article first published online: 12 AUG 2010
Copyright © 2010 Wiley-Liss, Inc.
Birth Defects Research Part A: Clinical and Molecular Teratology
Special Issue: 50th Anniversary of the Teratology Society
Volume 88, Issue 10, pages 827–837, October 2010
How to Cite
Thomas, J. D., Idrus, N. M., Monk, B. R. and Dominguez, H. D. (2010), Prenatal choline supplementation mitigates behavioral alterations associated with prenatal alcohol exposure in rats. Birth Defects Research Part A: Clinical and Molecular Teratology, 88: 827–837. doi: 10.1002/bdra.20713
- Issue published online: 13 OCT 2010
- Article first published online: 12 AUG 2010
- Manuscript Accepted: 15 JUN 2010
- Manuscript Revised: 20 MAY 2010
- Manuscript Received: 24 MAR 2010
- NIAAA. Grant Number: AA12446
- fetal alcohol spectrum disorders;
- fetal alcohol syndrome
Prenatal alcohol exposure can alter physical and behavioral development, leading to a range of fetal alcohol spectrum disorders. Despite warning labels, pregnant women continue to drink alcohol, creating a need to identify effective interventions to reduce the severity of alcohol's teratogenic effects. Choline is an essential nutrient that influences brain and behavioral development. Recent studies indicate that choline supplementation can reduce the teratogenic effects of developmental alcohol exposure. The present study examined whether choline supplementation during prenatal ethanol treatment could mitigate the adverse effects of ethanol on behavioral development.
Pregnant Sprague-Dawley rats were intubated with 6 g/kg/day ethanol in a binge-like manner from gestational days 5–20; pair-fed and ad libitum chow controls were included. During treatment, subjects from each group were intubated with either 250 mg/kg/day choline chloride or vehicle. Spontaneous alternation, parallel bar motor coordination, Morris water maze, and spatial working memory were assessed in male and female offspring.
Subjects prenatally exposed to alcohol exhibited delayed development of spontaneous alternation behavior and deficits on the working memory version of the Morris water maze during adulthood, effects that were mitigated with prenatal choline supplementation. Neither alcohol nor choline influenced performance on the motor coordination task.
These data indicate that choline supplementation during prenatal alcohol exposure may reduce the severity of fetal alcohol effects, particularly on alterations in tasks that require behavioral flexibility. These findings have important implications for children of women who drink alcohol during pregnancy. Birth Defects Research (Part A), 2010. © 2010 Wiley-Liss, Inc.