Female mice were exposed maternally to imazalil through diet to provide levels of 0 (control), 0.0006, 0.0018, and 0.0054% during gestation and lactation periods, and selected reproductive and neurobehavioral parameters were measured in F1 generation. There was no adverse effect of imazalil on litter size, litter weight, or sex ratio at birth. With respect to behavioral developmental parameters, surface righting on postnatal day 4 of male offspring was delayed significantly in a dose-related manner (p < 0.05). Regarding exploratory behavior in the F1 generation, movement time was significantly long (p = 0.0206) in the low-dose group of males at 8 weeks of age. Spontaneous behavior examination in males indicated that movement time increased but in females decreased in the low-dose groups in the F1 generation. The dose levels of imazalil in the present study produced some adverse effects in neurobehavioral parameters in mice.